Behavioural responses of freshwater planarians after short-term exposure to the insecticide chlorantraniliprole

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890388" target="_blank" >RIV/60076658:12520/16:43890388 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0166445X15300771" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0166445X15300771</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquatox.2015.10.018" target="_blank" >10.1016/j.aquatox.2015.10.018</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Behavioural responses of freshwater planarians after short-term exposure to the insecticide chlorantraniliprole

Popis výsledku v původním jazyce

Recent advances in video tracking technologies provide the tools for a sensitive and reproducible analysis of invertebrate activity under stressful conditions nurturing the field of behavioural ecotoxicology. This study aimed to evaluate behavioural responses of the freshwater planarian Dugesia subtentaculata exposed to a model compound, chlorantraniliprole (CAP). This compound is an anthranilic diamide insecticide and due to its neurotoxic action can, at low concentrations, impair behaviour of exposed organisms. Behavioural endpoints measured included feeding and locomotor activities. Feeding responses were based on planarian predatory behaviour using Chironomus riparius larvae as prey. Locomotion was measured by the traditional planarian locomotor velocity (pLMV) assay and additionally using an automated video tracking system using a Zebrabox (R) (Viewpoint, France) device. While feeding and pLMV were significantly impaired at 131.7 mu g/L CAP, the video tracking system showed that total distance covered by planarians was significantly reduced at concentrations as low as 26.2 mu g/L CAP. Our results show that more advanced automated video recording systems can be used in the development of sensitive bioassays allowing a reliable, time- and cost-effective quantification of behaviour in aquatic invertebrates. Due to their ecological relevance, behavioural responses should not be disregarded in risk assessment strategies and we advocate the suitability of planarians as suitable organisms for behavioural ecotoxicological studies.

Název v anglickém jazyce

Behavioural responses of freshwater planarians after short-term exposure to the insecticide chlorantraniliprole

Popis výsledku anglicky

Recent advances in video tracking technologies provide the tools for a sensitive and reproducible analysis of invertebrate activity under stressful conditions nurturing the field of behavioural ecotoxicology. This study aimed to evaluate behavioural responses of the freshwater planarian Dugesia subtentaculata exposed to a model compound, chlorantraniliprole (CAP). This compound is an anthranilic diamide insecticide and due to its neurotoxic action can, at low concentrations, impair behaviour of exposed organisms. Behavioural endpoints measured included feeding and locomotor activities. Feeding responses were based on planarian predatory behaviour using Chironomus riparius larvae as prey. Locomotion was measured by the traditional planarian locomotor velocity (pLMV) assay and additionally using an automated video tracking system using a Zebrabox (R) (Viewpoint, France) device. While feeding and pLMV were significantly impaired at 131.7 mu g/L CAP, the video tracking system showed that total distance covered by planarians was significantly reduced at concentrations as low as 26.2 mu g/L CAP. Our results show that more advanced automated video recording systems can be used in the development of sensitive bioassays allowing a reliable, time- and cost-effective quantification of behaviour in aquatic invertebrates. Due to their ecological relevance, behavioural responses should not be disregarded in risk assessment strategies and we advocate the suitability of planarians as suitable organisms for behavioural ecotoxicological studies.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DN - Vliv životního prostředí na zdraví

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquatic Toxicology

ISSN

0166-445X

e-ISSN

—

Svazek periodika

170

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

371-376

Kód UT WoS článku

000368564500040

EID výsledku v databázi Scopus

—