Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890396" target="_blank" >RIV/60076658:12520/16:43890396 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0044848615301368" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0044848615301368</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2015.08.017" target="_blank" >10.1016/j.aquaculture.2015.08.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies

Popis výsledku v původním jazyce

To identify the effects of oocyte ageing on egg quality in northern pike, Esox lucius, two experiments were performed as follows: in experiment I, partial volumes of the eggs from 7 pike females were stripped and fertilised with 2-day intervals for 8 days post-ovulation (DPO). In experiment II, the stripped eggs of 3 females were stored in ovarian fluid at 10 degrees C and fertilised separately with 12-hour intervals for 96 hours post-stripping (HPS). In both experiments, the eggs from each batch were fertilised with mixed milt obtained from 5 males. Eyeing, hatching and eyed-egg mortality rates as well as the occurrence of larval malformations and ploidy anomalies were considered as indices for egg quality. The results of experiment I indicated that the best and most reliable postovulatory stripping times in pike were at 2 DPO and up to 4 DPO, respectively, when females were retained at 10 degrees C. Over-ripening of the eggs occurred from 8 days after ovulation. The eyed-egg mortality and larval malformation rates did not show any significant increase up to 6 DPO, but then increased significantly and reached 36% and 43% at 8 DPO, respectively. The incidence of triploid larvae increased over time, from 0% at 0 and 2 DPO to 11% and 14% in more aged oocytes at 4 and 6 DPO, respectively. The second experiment clarified that the best post-stripping fertilisation time in pike was at 0 HPS. However, stored eggs in ovarian fluid could retain almost 50% of their initial fertilising ability for 24 h after stripping. Complete loss of egg viability occurred after 96 HPS, and the highest eyed-egg mortality rate (27%) and larval malformation rate (33%) were detected in this group. The obtained results suggest that for commercial artificial reproduction procedures in northern pike, the time interval between successive fish examinations for ovulation should not exceed 4 days.

Název v anglickém jazyce

Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies

Popis výsledku anglicky

To identify the effects of oocyte ageing on egg quality in northern pike, Esox lucius, two experiments were performed as follows: in experiment I, partial volumes of the eggs from 7 pike females were stripped and fertilised with 2-day intervals for 8 days post-ovulation (DPO). In experiment II, the stripped eggs of 3 females were stored in ovarian fluid at 10 degrees C and fertilised separately with 12-hour intervals for 96 hours post-stripping (HPS). In both experiments, the eggs from each batch were fertilised with mixed milt obtained from 5 males. Eyeing, hatching and eyed-egg mortality rates as well as the occurrence of larval malformations and ploidy anomalies were considered as indices for egg quality. The results of experiment I indicated that the best and most reliable postovulatory stripping times in pike were at 2 DPO and up to 4 DPO, respectively, when females were retained at 10 degrees C. Over-ripening of the eggs occurred from 8 days after ovulation. The eyed-egg mortality and larval malformation rates did not show any significant increase up to 6 DPO, but then increased significantly and reached 36% and 43% at 8 DPO, respectively. The incidence of triploid larvae increased over time, from 0% at 0 and 2 DPO to 11% and 14% in more aged oocytes at 4 and 6 DPO, respectively. The second experiment clarified that the best post-stripping fertilisation time in pike was at 0 HPS. However, stored eggs in ovarian fluid could retain almost 50% of their initial fertilising ability for 24 h after stripping. Complete loss of egg viability occurred after 96 HPS, and the highest eyed-egg mortality rate (27%) and larval malformation rate (33%) were detected in this group. The obtained results suggest that for commercial artificial reproduction procedures in northern pike, the time interval between successive fish examinations for ovulation should not exceed 4 days.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

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Svazek periodika

450

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

431-438

Kód UT WoS článku

000364854000058

EID výsledku v databázi Scopus

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