Triploidisation of pikeperch (Sander lucioperca), first success

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890479" target="_blank" >RIV/60076658:12520/16:43890479 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0044848616302642" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0044848616302642</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2016.05.016" target="_blank" >10.1016/j.aquaculture.2016.05.016</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Triploidisation of pikeperch (Sander lucioperca), first success

Popis výsledku v původním jazyce

The use of triploid fish in intensive aquaculture can lead to higher filet yield, faster growth and higher profit. To induce triploidy in pikeperch, fertilized eggs incubated at 15 degrees C were immersed in a water bath at shock temperatures (ST) of 29 degrees C (Exp. 1) and 31 degrees C (Exp. 2) at shock initiation times (TI) of 1, 3, 5, 7, and 10 min after egg and spermatozoa activation for 20 and 40 min. Ploidy levels of hatched larvae were determined by flow cytometry. Shock temperature of 29 degrees C led to low hatching rates (0-11.5%) and low rates of triploidy (0-75%). With ST of 31 degrees C, 100% triploidy was obtained with TI of 1 min and duration of 20 and 40 min and at TI of 5 min for 20 min. A shock temperature of 31 degrees C was associated with 0.9-74.1% malformed larvae compared to 0-3.3% at ST of 29 degrees C. The most important finding of this study is that heat shock treatment can produce all-triploid pikeperch population. However, further research is required to determine a method of production of all-triploid pikeperch population at higher hatching rates.

Název v anglickém jazyce

Triploidisation of pikeperch (Sander lucioperca), first success

Popis výsledku anglicky

The use of triploid fish in intensive aquaculture can lead to higher filet yield, faster growth and higher profit. To induce triploidy in pikeperch, fertilized eggs incubated at 15 degrees C were immersed in a water bath at shock temperatures (ST) of 29 degrees C (Exp. 1) and 31 degrees C (Exp. 2) at shock initiation times (TI) of 1, 3, 5, 7, and 10 min after egg and spermatozoa activation for 20 and 40 min. Ploidy levels of hatched larvae were determined by flow cytometry. Shock temperature of 29 degrees C led to low hatching rates (0-11.5%) and low rates of triploidy (0-75%). With ST of 31 degrees C, 100% triploidy was obtained with TI of 1 min and duration of 20 and 40 min and at TI of 5 min for 20 min. A shock temperature of 31 degrees C was associated with 0.9-74.1% malformed larvae compared to 0-3.3% at ST of 29 degrees C. The most important finding of this study is that heat shock treatment can produce all-triploid pikeperch population. However, further research is required to determine a method of production of all-triploid pikeperch population at higher hatching rates.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GL - Rybářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

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Svazek periodika

462

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

3

Strana od-do

115-117

Kód UT WoS článku

000377295900016

EID výsledku v databázi Scopus

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