Short-term storage of sterlet Acipenser ruthenus testicular cells at-80 degrees C

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43890505" target="_blank" >RIV/60076658:12520/16:43890505 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S001122401630013X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S001122401630013X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cryobiol.2016.03.002" target="_blank" >10.1016/j.cryobiol.2016.03.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Short-term storage of sterlet Acipenser ruthenus testicular cells at-80 degrees C

Popis výsledku v původním jazyce

The conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 degrees C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and 1.5 M ethylene glycol as a cryoprotectant, chilled from 10 to -80 degrees C at a cooling rate of -1 degrees C per min, and stored under varying conditions. Our results revealed a significant effect of storage conditions on the number of living and dead cells (p > 0.05). Samples that were stored for 7 days at -80 degrees C showed a considerable decline in terms of cell viability compared to samples stored for 2 days storage at -80 degrees C or in LN. This result indicated that testicular cells can be stored at -80 degrees C and/or on dry ice, for 2 days with minimum loss of viability.

Název v anglickém jazyce

Short-term storage of sterlet Acipenser ruthenus testicular cells at-80 degrees C

Popis výsledku anglicky

The conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. This study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 degrees C for purpose of a short-term storage in a deep freezer or shipment on dried ice. Testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mM glucose, and 1.5 M ethylene glycol as a cryoprotectant, chilled from 10 to -80 degrees C at a cooling rate of -1 degrees C per min, and stored under varying conditions. Our results revealed a significant effect of storage conditions on the number of living and dead cells (p > 0.05). Samples that were stored for 7 days at -80 degrees C showed a considerable decline in terms of cell viability compared to samples stored for 2 days storage at -80 degrees C or in LN. This result indicated that testicular cells can be stored at -80 degrees C and/or on dry ice, for 2 days with minimum loss of viability.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cryobiology

ISSN

0011-2240

e-ISSN

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Svazek periodika

72

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

3

Strana od-do

154-156

Kód UT WoS článku

000374078600012

EID výsledku v databázi Scopus

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