The effect of cypermethrin on oxidative stres and antioxidant biomarkers in marbled crayfish (Procambarus fallax F. virginalis)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F16%3A43892087" target="_blank" >RIV/60076658:12520/16:43892087 - isvavai.cz</a>

Výsledek na webu

<a href="http://goo.gl/wM2FHc" target="_blank" >http://goo.gl/wM2FHc</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of cypermethrin on oxidative stres and antioxidant biomarkers in marbled crayfish (Procambarus fallax F. virginalis)

Popis výsledku v původním jazyce

OBJECTIVES: Pyrethroid insecticides are known to be highly toxic to non-target aquatic organisms. Toxic effects of different types of pyrethroids to fish has been well described but there is a dearth of data on their effects to aquatic crustaceans. The aim of this study was to assess the effects of a commercial product Cyperkill 25 EC (25% of cypermethrin as an active substance) on oxidative stress and antioxidants activity in marbled crayfish. DESIGN: The juvenile crayfish were exposed to two concentrations of Cyperkill 25 EC: 0.02 ?g.L-1 (10% 96hLC50) and 0.05 ?g.L-1 (30% 96hLC50) for 72 hours. Activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione-S-transpherase (GST) and reduced glutathione (GSH), as well as levels of thiobarbituric acid reactive substances (TBARS) were assessed in the whole-body homogenate. RESULTS: Cyperkill 25 EC in both tested concentrations caused significant (p&lt;0.01) decreased in levels of TBARS. Changes in the antioxidants activity were observed in every examined parameters except for the catalase. Cyperkill 25 EC in concentration 0.05 ?g.L-1 caused significant increase (p&lt;0.05) of SOD activity and significant decrease of GST (p&lt;0.01) and GSH (p&lt;0.05) activity. The activity of GR was significantly (p&lt;0.01) decreased in both treated groups. CONCLUSIONS: This study suggests that Cyperkill 25 EC causes oxidative stress and also disruption of antioxidant systems in crayfish. Expanding to our previous knowledge, obtained results describe effects of cypermethrin also to non-target aquatic invertebrates and suggest a need of deeper understanding of mechanisms involved in this process.

Název v anglickém jazyce

The effect of cypermethrin on oxidative stres and antioxidant biomarkers in marbled crayfish (Procambarus fallax F. virginalis)

Popis výsledku anglicky

OBJECTIVES: Pyrethroid insecticides are known to be highly toxic to non-target aquatic organisms. Toxic effects of different types of pyrethroids to fish has been well described but there is a dearth of data on their effects to aquatic crustaceans. The aim of this study was to assess the effects of a commercial product Cyperkill 25 EC (25% of cypermethrin as an active substance) on oxidative stress and antioxidants activity in marbled crayfish. DESIGN: The juvenile crayfish were exposed to two concentrations of Cyperkill 25 EC: 0.02 ?g.L-1 (10% 96hLC50) and 0.05 ?g.L-1 (30% 96hLC50) for 72 hours. Activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione-S-transpherase (GST) and reduced glutathione (GSH), as well as levels of thiobarbituric acid reactive substances (TBARS) were assessed in the whole-body homogenate. RESULTS: Cyperkill 25 EC in both tested concentrations caused significant (p&lt;0.01) decreased in levels of TBARS. Changes in the antioxidants activity were observed in every examined parameters except for the catalase. Cyperkill 25 EC in concentration 0.05 ?g.L-1 caused significant increase (p&lt;0.05) of SOD activity and significant decrease of GST (p&lt;0.01) and GSH (p&lt;0.05) activity. The activity of GR was significantly (p&lt;0.01) decreased in both treated groups. CONCLUSIONS: This study suggests that Cyperkill 25 EC causes oxidative stress and also disruption of antioxidant systems in crayfish. Expanding to our previous knowledge, obtained results describe effects of cypermethrin also to non-target aquatic invertebrates and suggest a need of deeper understanding of mechanisms involved in this process.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neuroendocrinology Letters

ISSN

0172-780X

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

Suppl. 1

Stát vydavatele periodika

SE - Švédské království

Počet stran výsledku

7

Strana od-do

53-59

Kód UT WoS článku

000406112100010

EID výsledku v databázi Scopus

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