Cryopreservation of Carp (Cyprinus carpio L.) Sperm: Impact of Seeding and Freezing Rates on Post-Thaw Outputs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F17%3A43895335" target="_blank" >RIV/60076658:12520/17:43895335 - isvavai.cz</a>

Výsledek na webu

<a href="http://online.liebertpub.com/doi/10.1089/bio.2016.0065" target="_blank" >http://online.liebertpub.com/doi/10.1089/bio.2016.0065</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/bio.2016.0065" target="_blank" >10.1089/bio.2016.0065</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cryopreservation of Carp (Cyprinus carpio L.) Sperm: Impact of Seeding and Freezing Rates on Post-Thaw Outputs

Popis výsledku v původním jazyce

In the present study, we examined various freezing protocols, effects of controlled seeding, and changes in cooling rate and determined the endpoint (temperature at which sample could be plugged into liquid nitrogen (LN) without visible effect on survival rate after thawing) to reveal the relative importance of each different stage of cooling on freezing success during cryobanking of carp sperm. Sperm samples from different individual carp males were frozen in 0.5 mL straws by conventional freezing. Cooling rates were determined by monitoring the sample&apos;s internal temperature. We compared four freezing protocols, which involved placing sperm samples at various levels (1, 3, 6, and 9cm) above the LN surface (corresponding to -190 degrees C, -150 degrees C, -110 degrees C, and -70 degrees C, respectively) for 20 minutes followed by transferring the samples into LN. Freezing at 3cm above the LN surface resulted in the highest motility (33%+/- 8%) and velocity (118 +/- 9 mu/s) of spermatozoa after thawing and diluting in swimming medium. We determined that -90 degrees C is an optimal temperature at which immersing the samples in LN does not affect sperm motility after thawing and shorten the process of freezing for around three times. Motility of spermatozoa cryopreserved with or without a seeding procedure was not significantly different after thawing. Therefore, we hypothesize that supercooling the sample during the conventional freezing procedure is not the main damaging factor during carp spermatozoa cryopreservation. However, the cooling rate itself is important, because it determines the ability of the sperm to dehydrate and survive cryopreservation.

Název v anglickém jazyce

Cryopreservation of Carp (Cyprinus carpio L.) Sperm: Impact of Seeding and Freezing Rates on Post-Thaw Outputs

Popis výsledku anglicky

In the present study, we examined various freezing protocols, effects of controlled seeding, and changes in cooling rate and determined the endpoint (temperature at which sample could be plugged into liquid nitrogen (LN) without visible effect on survival rate after thawing) to reveal the relative importance of each different stage of cooling on freezing success during cryobanking of carp sperm. Sperm samples from different individual carp males were frozen in 0.5 mL straws by conventional freezing. Cooling rates were determined by monitoring the sample&apos;s internal temperature. We compared four freezing protocols, which involved placing sperm samples at various levels (1, 3, 6, and 9cm) above the LN surface (corresponding to -190 degrees C, -150 degrees C, -110 degrees C, and -70 degrees C, respectively) for 20 minutes followed by transferring the samples into LN. Freezing at 3cm above the LN surface resulted in the highest motility (33%+/- 8%) and velocity (118 +/- 9 mu/s) of spermatozoa after thawing and diluting in swimming medium. We determined that -90 degrees C is an optimal temperature at which immersing the samples in LN does not affect sperm motility after thawing and shorten the process of freezing for around three times. Motility of spermatozoa cryopreserved with or without a seeding procedure was not significantly different after thawing. Therefore, we hypothesize that supercooling the sample during the conventional freezing procedure is not the main damaging factor during carp spermatozoa cryopreservation. However, the cooling rate itself is important, because it determines the ability of the sperm to dehydrate and survive cryopreservation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biopreservation and Biobanking

ISSN

1947-5535

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

234-240

Kód UT WoS článku

000402710100010

EID výsledku v databázi Scopus

2-s2.0-85020213092