Insight into the modulation of intestinal proteome of juvenile common carp (Cyprinus carpio L.) after dietary exposure to ZnO nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F18%3A43896112" target="_blank" >RIV/60076658:12520/18:43896112 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0048969717321277?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0048969717321277?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scitotenv.2017.08.129" target="_blank" >10.1016/j.scitotenv.2017.08.129</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Insight into the modulation of intestinal proteome of juvenile common carp (Cyprinus carpio L.) after dietary exposure to ZnO nanoparticles

Popis výsledku v původním jazyce

ZnO nanoparticles (NPs) are widely used in industrial and consumer products. Therefore understanding their interaction with biological systems is key to their safe application. Proteomics was applied to assess the sub-lethal effects of dietary ZnO NPs on two parts of carp intestine, the intestinal folds and the muscular parts. A commercial carp feed containing 500mg kg(-1) of ZnO NPs was fed to fish for six weeks. The abundances of 32 proteins in the treated intestinal folds were significantly changed and in addition, 28 proteins were significantly changed in the muscular parts. Pathways analysis revealed downregulation of pathways attributed to protein synthesis in both parts of the treated intestine. Remodelling of actin cytoskeleton pathways were regulated positively and negatively in intestinal folds and muscular parts, respectively, albeit via different mechanisms. Apoptosis response was indicated in exposed intestinal folds, whereas elevated levels of protein associated with cancerous cell survival were observed in the muscular parts. Results showed that ZnO NPs affected the protein abundances associated with cell motility, immune system response, oxidative stress response, as well as cell metabolism. Data are available via ProteomeXchange with identifier PXD006867.

Název v anglickém jazyce

Insight into the modulation of intestinal proteome of juvenile common carp (Cyprinus carpio L.) after dietary exposure to ZnO nanoparticles

Popis výsledku anglicky

ZnO nanoparticles (NPs) are widely used in industrial and consumer products. Therefore understanding their interaction with biological systems is key to their safe application. Proteomics was applied to assess the sub-lethal effects of dietary ZnO NPs on two parts of carp intestine, the intestinal folds and the muscular parts. A commercial carp feed containing 500mg kg(-1) of ZnO NPs was fed to fish for six weeks. The abundances of 32 proteins in the treated intestinal folds were significantly changed and in addition, 28 proteins were significantly changed in the muscular parts. Pathways analysis revealed downregulation of pathways attributed to protein synthesis in both parts of the treated intestine. Remodelling of actin cytoskeleton pathways were regulated positively and negatively in intestinal folds and muscular parts, respectively, albeit via different mechanisms. Apoptosis response was indicated in exposed intestinal folds, whereas elevated levels of protein associated with cancerous cell survival were observed in the muscular parts. Results showed that ZnO NPs affected the protein abundances associated with cell motility, immune system response, oxidative stress response, as well as cell metabolism. Data are available via ProteomeXchange with identifier PXD006867.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Science of the Total Environment

ISSN

0048-9697

e-ISSN

—

Svazek periodika

613

Číslo periodika v rámci svazku

2/2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

62-71

Kód UT WoS článku

000414160500008

EID výsledku v databázi Scopus

2-s2.0-85028955553