Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F18%3A43897275" target="_blank" >RIV/60076658:12520/18:43897275 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0378432017310801" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378432017310801</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.anireprosci.2018.03.027" target="_blank" >10.1016/j.anireprosci.2018.03.027</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm

Popis výsledku v původním jazyce

Heat Shock Protein 90 (HSP90) is a fertility-associated protein, the expression of which positively correlates with sperm quality in many species. Geranylgeranylacetone (GGA) is reported to induce expression of HSP90. The present study aimed to investigate whether GGA induced expression of HSP90 in Acipenser sinensis sperm to exert a cryoprotective effect. Sperm from five male A. sinensis was combined with extender containing 20 mmol/L tris pH = 8.1, 10% v/v methanol, 2-5 mmol/L KC1, 15 mmol/L lactose, and 15 mmol/L trehalose, with GGA at 0, 14, 67, 135, 673, 1346, or 6731 mu mol/L. After cryopreservation and thawing, the percentage of motile spermatozoa, spermatozoon curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), acrosome integrity, and membrane integrity, as well as fertility were evaluated. Sperm quality increased with the increase of GGA to 673 mu mol/L, but decreased at higher concentrations. Expression levels of HSP90 alpha were detected by Western blot in sperm frozen with GGA at 673 mu mol/L (highest obtained sperm quality), 6731 mu mol/L (highest GGA concentration), and a control without GGA. The expression of HSP90 alpha increased with the increase in GGA, with lowest expression observed in the control. GGA was found to induce increase of HSP90 alpha, and this increase was associated with higher quality cryopreserved sperm at concentrations 5.673 mu mol/L. This research suggests a viable technique to increase the quality of cryopreserved A. sinensis sperm by adding GGA to induce expression of HSP90 alpha.

Název v anglickém jazyce

Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm

Popis výsledku anglicky

Heat Shock Protein 90 (HSP90) is a fertility-associated protein, the expression of which positively correlates with sperm quality in many species. Geranylgeranylacetone (GGA) is reported to induce expression of HSP90. The present study aimed to investigate whether GGA induced expression of HSP90 in Acipenser sinensis sperm to exert a cryoprotective effect. Sperm from five male A. sinensis was combined with extender containing 20 mmol/L tris pH = 8.1, 10% v/v methanol, 2-5 mmol/L KC1, 15 mmol/L lactose, and 15 mmol/L trehalose, with GGA at 0, 14, 67, 135, 673, 1346, or 6731 mu mol/L. After cryopreservation and thawing, the percentage of motile spermatozoa, spermatozoon curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), acrosome integrity, and membrane integrity, as well as fertility were evaluated. Sperm quality increased with the increase of GGA to 673 mu mol/L, but decreased at higher concentrations. Expression levels of HSP90 alpha were detected by Western blot in sperm frozen with GGA at 673 mu mol/L (highest obtained sperm quality), 6731 mu mol/L (highest GGA concentration), and a control without GGA. The expression of HSP90 alpha increased with the increase in GGA, with lowest expression observed in the control. GGA was found to induce increase of HSP90 alpha, and this increase was associated with higher quality cryopreserved sperm at concentrations 5.673 mu mol/L. This research suggests a viable technique to increase the quality of cryopreserved A. sinensis sperm by adding GGA to induce expression of HSP90 alpha.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animal Reproduction Science

ISSN

0378-4320

e-ISSN

—

Svazek periodika

193

Číslo periodika v rámci svazku

JUN 2018

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

19-25

Kód UT WoS článku

000433999000003

EID výsledku v databázi Scopus

2-s2.0-85046348801