The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F19%3A43899034" target="_blank" >RIV/60076658:12520/19:43899034 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/19:00503711 RIV/61988987:17310/19:A21025N6

Výsledek na webu

<a href="http://trjfas.org/uploads/pdf_1328.pdf" target="_blank" >http://trjfas.org/uploads/pdf_1328.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4194/1303-2712-v19_2_09" target="_blank" >10.4194/1303-2712-v19_2_09</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid

Popis výsledku v původním jazyce

Detailed knowledge of early embryo development is a prerequisite for implementation of reproductive biotechnologies. We described the timing of mid-blastula transition in the sturgeon Acipenser ruthenus and hybrid of A. ruthenus and Acipenser gueldenstaedtii. To identify mitotic changes during cell division, the nuclei of embryo blastomeres were sampled every 30 min from 4 to 25 h post-fertilization and stained with 5 mg/ml 4&apos;-6-diaminido-2phenylindole. We observed embryos of hybrids to lose synchrony after the eighth cell division, one cycle earlier than in purebred sterlet embryos. To reveal the timing of zygotic gene activation, developing embryos were dechorionated and injected with 10, 50, 100, 250, or 500 mu g/ml a-Amanitin. Termination of development in embryos was recorded throughout the period from the 1000 cell stage until gastrulation, showing the process of transition from maternal to zygotic gene transcription in sterlet to be of extended duration and not directly linked to mid-blastula transition.

Název v anglickém jazyce

The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid

Popis výsledku anglicky

Detailed knowledge of early embryo development is a prerequisite for implementation of reproductive biotechnologies. We described the timing of mid-blastula transition in the sturgeon Acipenser ruthenus and hybrid of A. ruthenus and Acipenser gueldenstaedtii. To identify mitotic changes during cell division, the nuclei of embryo blastomeres were sampled every 30 min from 4 to 25 h post-fertilization and stained with 5 mg/ml 4&apos;-6-diaminido-2phenylindole. We observed embryos of hybrids to lose synchrony after the eighth cell division, one cycle earlier than in purebred sterlet embryos. To reveal the timing of zygotic gene activation, developing embryos were dechorionated and injected with 10, 50, 100, 250, or 500 mu g/ml a-Amanitin. Termination of development in embryos was recorded throughout the period from the 1000 cell stage until gastrulation, showing the process of transition from maternal to zygotic gene transcription in sterlet to be of extended duration and not directly linked to mid-blastula transition.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Turkish Journal of Fisheries and Aquatic Sciences

ISSN

1303-2712

e-ISSN

—

Svazek periodika

19

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

TR - Turecká republika

Počet stran výsledku

8

Strana od-do

77-84

Kód UT WoS článku

000443957500009

EID výsledku v databázi Scopus

2-s2.0-85053340233