Swimming at different temperatures: The lipid composition of sperm from three freshwater fish species determined by mass spectrometry and nuclear magnetic resonance spectroscopy
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F19%3A43899210" target="_blank" >RIV/60076658:12520/19:43899210 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S0009308419300453" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0009308419300453</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chemphyslip.2019.03.014" target="_blank" >10.1016/j.chemphyslip.2019.03.014</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Swimming at different temperatures: The lipid composition of sperm from three freshwater fish species determined by mass spectrometry and nuclear magnetic resonance spectroscopy
Popis výsledku v původním jazyce
The spawning behavior of different fish species is as diverse as their habitats. A lot of factors influence the (phospho)lipid composition of fish sperm, including the water temperature at which spawning takes place. Therefore, this study aimed on the elucidation of the phospholipid composition of sperm from three fish species from different orders (common carp - Cyprinus carpio, northern pike - Esox lucius and burbot - Lota lota) with different spawning temperatures by nuclear magnetic resonance spectroscopy (NMR), matrix-assisted laser desorption/ionization mass spectrometry and thin-layer chromatography (TLC) coupled to electrospray ionization mass spectrometry as well as gas chromatography. Next to the lipid composition that was different for carp, northern pike and burbot, regarding the moieties of the different (phospho)lipid classes (particularly sphingomyelin and acidic phospholipids) and the saturation degree of the fatty acyl residues, there were differences observed depending on the analytical method that was used. The results from TLC and NMR investigations differed regarding the amounts of the different phospholipids. Reasons for these discrepancies are discussed in detail.
Název v anglickém jazyce
Swimming at different temperatures: The lipid composition of sperm from three freshwater fish species determined by mass spectrometry and nuclear magnetic resonance spectroscopy
Popis výsledku anglicky
The spawning behavior of different fish species is as diverse as their habitats. A lot of factors influence the (phospho)lipid composition of fish sperm, including the water temperature at which spawning takes place. Therefore, this study aimed on the elucidation of the phospholipid composition of sperm from three fish species from different orders (common carp - Cyprinus carpio, northern pike - Esox lucius and burbot - Lota lota) with different spawning temperatures by nuclear magnetic resonance spectroscopy (NMR), matrix-assisted laser desorption/ionization mass spectrometry and thin-layer chromatography (TLC) coupled to electrospray ionization mass spectrometry as well as gas chromatography. Next to the lipid composition that was different for carp, northern pike and burbot, regarding the moieties of the different (phospho)lipid classes (particularly sphingomyelin and acidic phospholipids) and the saturation degree of the fatty acyl residues, there were differences observed depending on the analytical method that was used. The results from TLC and NMR investigations differed regarding the amounts of the different phospholipids. Reasons for these discrepancies are discussed in detail.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10604 - Reproductive biology (medical aspects to be 3)
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Chemistry and Physics of Lipids
ISSN
0009-3084
e-ISSN
—
Svazek periodika
221
Číslo periodika v rámci svazku
neuveden
Stát vydavatele periodika
IE - Irsko
Počet stran výsledku
8
Strana od-do
65-72
Kód UT WoS článku
000468718000009
EID výsledku v databázi Scopus
2-s2.0-85063536681