Optimized application of rotifers Brachionus plicatilis for rearing pikeperch Sander lucioperca L. larvae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F19%3A43899237" target="_blank" >RIV/60076658:12520/19:43899237 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10499-019-00420-3" target="_blank" >https://link.springer.com/article/10.1007/s10499-019-00420-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10499-019-00420-3" target="_blank" >10.1007/s10499-019-00420-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Optimized application of rotifers Brachionus plicatilis for rearing pikeperch Sander lucioperca L. larvae

Popis výsledku v původním jazyce

This study aimed to optimize the use of euryhaline rotifer Brachionus plicatilis for rearing larval pikeperch Sander lucioperca L. We assessed motility of rotifers under salinities of 0 parts per thousand, 2 parts per thousand, 4 parts per thousand, 8 parts per thousand, and 16 parts per thousand over a 6-h period. Rotifers stocked into freshwater were completely immotile within 2 h. The motility of rotifers after 6 h was 10 +/- 2.27% at 2 parts per thousand, 35 +/- 5.58% at 4 parts per thousand, 79 +/- 30.35% at 8 parts per thousand, and 92 +/- 1.98% at 16 parts per thousand. A second trial quantified the effect of the target salinity levels on pikeperch gut fullness over the course of 11 h. The gut fullness of larvae fed with rotifers was significantly lower at 8 parts per thousand salinity than in other tested groups. Salinity of 16 parts per thousand resulted in 100% mortality. Survival and growth of pikeperch larvae from 4 to 11 days post-hatching (DPH) was analyzed at low (2 parts per thousand and 4 parts per thousand) and medium (8 parts per thousand) salinity. Differences in larval performance under tested salinities were found in survival (S), total length, SGR, eye diameter, myotome height, and gut fullness. The highest mean survival (S) and specific growth rate (SGR) were obtained at 2 parts per thousand (S = 64%; SGR = 10 +/- 5.4% day(-1)) and 4 parts per thousand (S = 65%; SGR = 8.23 +/- 3.4% day(-1)) salinity and significantly differed from 8 parts per thousand (S = 36%; SGR = 5 +/- 2.3% day(-1)) and control (S = 34%; SGR = 7 +/- 3% day(-1)) at 11 DPH. At the conclusion of the trial (11 DPH), larvae of all exposures and control presented normal histology with no signs of pathology. Morphometric analyses revealed significant differences in brush border and fold height of anterior intestine among treatment groups. Larvae at 2 parts per thousand salinity showed significantly higher intestinal fold height (31.2 +/- 5.40 mu m) compared to those at 4 parts per thousand (26.5 +/- 6.11 mu m), 8 parts per thousand (26.3 +/- 4.52 mu m), and control (22.8 +/- 5.13 mu m) (p ? 0.05). Larvae at 4 parts per thousand salinity had significantly higher brush border height (2.50 +/- 0.73 mu m) compared to those at 2 parts per thousand (2.27 +/- 0.63 mu m) and 8 parts per thousand (1.87 +/- 0.44 mu m) salinity and control (1.66 +/- 0.56 mu m) (p &lt; 0.05). The results showed 2 parts per thousand and 4 parts per thousand salinity supported higher numbers of motile B. plicatilis and higher larval survival and growth rate to 11 DPH.

Název v anglickém jazyce

Optimized application of rotifers Brachionus plicatilis for rearing pikeperch Sander lucioperca L. larvae

Popis výsledku anglicky

This study aimed to optimize the use of euryhaline rotifer Brachionus plicatilis for rearing larval pikeperch Sander lucioperca L. We assessed motility of rotifers under salinities of 0 parts per thousand, 2 parts per thousand, 4 parts per thousand, 8 parts per thousand, and 16 parts per thousand over a 6-h period. Rotifers stocked into freshwater were completely immotile within 2 h. The motility of rotifers after 6 h was 10 +/- 2.27% at 2 parts per thousand, 35 +/- 5.58% at 4 parts per thousand, 79 +/- 30.35% at 8 parts per thousand, and 92 +/- 1.98% at 16 parts per thousand. A second trial quantified the effect of the target salinity levels on pikeperch gut fullness over the course of 11 h. The gut fullness of larvae fed with rotifers was significantly lower at 8 parts per thousand salinity than in other tested groups. Salinity of 16 parts per thousand resulted in 100% mortality. Survival and growth of pikeperch larvae from 4 to 11 days post-hatching (DPH) was analyzed at low (2 parts per thousand and 4 parts per thousand) and medium (8 parts per thousand) salinity. Differences in larval performance under tested salinities were found in survival (S), total length, SGR, eye diameter, myotome height, and gut fullness. The highest mean survival (S) and specific growth rate (SGR) were obtained at 2 parts per thousand (S = 64%; SGR = 10 +/- 5.4% day(-1)) and 4 parts per thousand (S = 65%; SGR = 8.23 +/- 3.4% day(-1)) salinity and significantly differed from 8 parts per thousand (S = 36%; SGR = 5 +/- 2.3% day(-1)) and control (S = 34%; SGR = 7 +/- 3% day(-1)) at 11 DPH. At the conclusion of the trial (11 DPH), larvae of all exposures and control presented normal histology with no signs of pathology. Morphometric analyses revealed significant differences in brush border and fold height of anterior intestine among treatment groups. Larvae at 2 parts per thousand salinity showed significantly higher intestinal fold height (31.2 +/- 5.40 mu m) compared to those at 4 parts per thousand (26.5 +/- 6.11 mu m), 8 parts per thousand (26.3 +/- 4.52 mu m), and control (22.8 +/- 5.13 mu m) (p ? 0.05). Larvae at 4 parts per thousand salinity had significantly higher brush border height (2.50 +/- 0.73 mu m) compared to those at 2 parts per thousand (2.27 +/- 0.63 mu m) and 8 parts per thousand (1.87 +/- 0.44 mu m) salinity and control (1.66 +/- 0.56 mu m) (p &lt; 0.05). The results showed 2 parts per thousand and 4 parts per thousand salinity supported higher numbers of motile B. plicatilis and higher larval survival and growth rate to 11 DPH.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture International

ISSN

0967-6120

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

13

Strana od-do

1137-1149

Kód UT WoS článku

000476712200019

EID výsledku v databázi Scopus

2-s2.0-85068859518