Interleukin-1 beta induces autophagy of mouse preimplantation embryos and improves blastocyst quality

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900802" target="_blank" >RIV/60076658:12520/20:43900802 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1002/jcb.29345" target="_blank" >https://doi.org/10.1002/jcb.29345</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jcb.29345" target="_blank" >10.1002/jcb.29345</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interleukin-1 beta induces autophagy of mouse preimplantation embryos and improves blastocyst quality

Popis výsledku v původním jazyce

Autophagy is one of the basic cellular mechanism during preimplantation development of mammalian embryos, and it plays crucial role in several physiological processes. It is induced by interleukin (IL)-1 beta in mammalian cells. Our present study shows that IL-1 beta is important for autophagy activation in embryo development. Our in vitro culture system analysis shows effect of IL-1 beta in medium on the development of mouse embryos and it was found to be concentration dependent. A preimplantation embryo culture using medium containing IL-1 beta did not improve cleavage and blastocyst development rates of mouse embryos; however, blastocyst quality was significantly improved by increasing total cell number, especially in supplementary 20 ng/mL IL-1 beta. Furthermore, autophagy activation mainly occurs in 2 to 4 cell embryo and blastocyst, 20 ng/mL IL-1 beta into culture medium can effectively enhance levels of messenger RNA and protein of autophagy-related-factors in 2 to 4 cell embryos and blastocyst, while these factors reduce in VGX-1027 (IL-1 beta inhibitor) groups that also reduce the quality of blastocyst. Effects of IL-1 beta on the development of embryo reduced in 20 ng/mL IL-1 beta supplemented group when 5 mM 3-methyladenine (3-MA) was also added, which used to inhibit autophagy activation in endogenous PtdIns3Ks signal pathway. Our current results show that exogenous IL-1 beta can effectively induce autophagy in mouse embryos at stages of 2 to 8 cell and blastocyst, that also help to improve the quality of blastocyst.

Název v anglickém jazyce

Interleukin-1 beta induces autophagy of mouse preimplantation embryos and improves blastocyst quality

Popis výsledku anglicky

Autophagy is one of the basic cellular mechanism during preimplantation development of mammalian embryos, and it plays crucial role in several physiological processes. It is induced by interleukin (IL)-1 beta in mammalian cells. Our present study shows that IL-1 beta is important for autophagy activation in embryo development. Our in vitro culture system analysis shows effect of IL-1 beta in medium on the development of mouse embryos and it was found to be concentration dependent. A preimplantation embryo culture using medium containing IL-1 beta did not improve cleavage and blastocyst development rates of mouse embryos; however, blastocyst quality was significantly improved by increasing total cell number, especially in supplementary 20 ng/mL IL-1 beta. Furthermore, autophagy activation mainly occurs in 2 to 4 cell embryo and blastocyst, 20 ng/mL IL-1 beta into culture medium can effectively enhance levels of messenger RNA and protein of autophagy-related-factors in 2 to 4 cell embryos and blastocyst, while these factors reduce in VGX-1027 (IL-1 beta inhibitor) groups that also reduce the quality of blastocyst. Effects of IL-1 beta on the development of embryo reduced in 20 ng/mL IL-1 beta supplemented group when 5 mM 3-methyladenine (3-MA) was also added, which used to inhibit autophagy activation in endogenous PtdIns3Ks signal pathway. Our current results show that exogenous IL-1 beta can effectively induce autophagy in mouse embryos at stages of 2 to 8 cell and blastocyst, that also help to improve the quality of blastocyst.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Cellular Biochemistry

ISSN

0730-2312

e-ISSN

—

Svazek periodika

121

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

1087-1100

Kód UT WoS článku

000483802600001

EID výsledku v databázi Scopus

2-s2.0-85071632348