Effects of Bacillus subtilis supplementation in soybean meal-based diets on growth performance, diet digestibility and gut health in bullfrog Lithobates catesbeianus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900833" target="_blank" >RIV/60076658:12520/20:43900833 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aqrep.2020.100273" target="_blank" >https://doi.org/10.1016/j.aqrep.2020.100273</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aqrep.2020.100273" target="_blank" >10.1016/j.aqrep.2020.100273</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of Bacillus subtilis supplementation in soybean meal-based diets on growth performance, diet digestibility and gut health in bullfrog Lithobates catesbeianus

Popis výsledku v původním jazyce

An 8-week feeding experiment was carried out to evaluate the supplemental effects of Bacillus subtilis in all-plant protein diets on growth, nutrients digestibility, and intestinal histomorphology and microbial composition in bullfrog Lithobates catesbeianus. Totally four diets were prepared: a non-supplemented control diet and three probiotic diets containing 1 x 10(5), 1 x 10(7) or 1 x 10(9) CFU g(-1) of B. subtilis (Con, BS5, BS7 and BS9 diets). The experiment was done in triplicates and bullfrogs averaging 42.5 +/- 0.2 g were fed to visual satiety twice daily. No significant changes in growth, feed utilization, body composition and survival rate were found by probiotic application (P &gt; 0.05). However, significant improvements in apparent digestibility coefficients of protein, calcium and phosphorus were found by probiotic supplementation. Moreover, whole-body phosphorous and calcium contents were enhanced by probiotic administration. The highest villus height and thickness were recorded in the BS5 group. Operational taxonomic unit (OTU) diversity and richness significantly increased by application of 1 x 10(7) CFU g(-1) of B. subtilis. Furthermore, B. subtilis abundance in the gut was enhanced with increasing the probiotic inclusion level indicating the successful establishment of the probiotic. The findings in this research showed that supplementing 1 x 10(7) CFU g(-1) of B. subtilis in soybean meal-based diets for bullfrog beneficially influences nutrients digestibility and uptake, and intestinal structure and microbial composition.

Název v anglickém jazyce

Effects of Bacillus subtilis supplementation in soybean meal-based diets on growth performance, diet digestibility and gut health in bullfrog Lithobates catesbeianus

Popis výsledku anglicky

An 8-week feeding experiment was carried out to evaluate the supplemental effects of Bacillus subtilis in all-plant protein diets on growth, nutrients digestibility, and intestinal histomorphology and microbial composition in bullfrog Lithobates catesbeianus. Totally four diets were prepared: a non-supplemented control diet and three probiotic diets containing 1 x 10(5), 1 x 10(7) or 1 x 10(9) CFU g(-1) of B. subtilis (Con, BS5, BS7 and BS9 diets). The experiment was done in triplicates and bullfrogs averaging 42.5 +/- 0.2 g were fed to visual satiety twice daily. No significant changes in growth, feed utilization, body composition and survival rate were found by probiotic application (P &gt; 0.05). However, significant improvements in apparent digestibility coefficients of protein, calcium and phosphorus were found by probiotic supplementation. Moreover, whole-body phosphorous and calcium contents were enhanced by probiotic administration. The highest villus height and thickness were recorded in the BS5 group. Operational taxonomic unit (OTU) diversity and richness significantly increased by application of 1 x 10(7) CFU g(-1) of B. subtilis. Furthermore, B. subtilis abundance in the gut was enhanced with increasing the probiotic inclusion level indicating the successful establishment of the probiotic. The findings in this research showed that supplementing 1 x 10(7) CFU g(-1) of B. subtilis in soybean meal-based diets for bullfrog beneficially influences nutrients digestibility and uptake, and intestinal structure and microbial composition.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture Reports

ISSN

2352-5134

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000517752000039

EID výsledku v databázi Scopus

2-s2.0-85078053587