Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900931" target="_blank" >RIV/60076658:12520/20:43900931 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s10695-020-00805-9" target="_blank" >https://doi.org/10.1007/s10695-020-00805-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-020-00805-9" target="_blank" >10.1007/s10695-020-00805-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation

Popis výsledku v původním jazyce

This study aimed to investigate the effect of fractionated seminal plasma on characteristics of common carp Cyprinus carpio cryopreserved sperm. Nanosep (R) centrifugal devices yielded four seminal plasma fractions with different total protein content ranging in molecular weight from less than 17 to almost 74 kDa. Each protein fraction was added to semen extender medium prior to freezing. Spermatozoon motility characteristics and DNA integrity were analyzed in supplemented and non-supplemented cryopreserved samples. The cryopreservation process strongly affected the swim-up sperm quality. Treatment with fractions 1, 2, 3, and 4 was associated with significantly higher spermatozoon motility rate and curvilinear velocity than seen in extender only, with highest values obtained with fraction 4 (78.21 +/- 2.41% and 168.05 +/- 4.46 mu m/s, respectively). Significantly less DNA damage, expressed as percent tail DNA (12.23 +/- 1.27) and olive tail moment (0.68 +/- 0.12), was recorded in fraction 4. The findings indicated that addition of fractionated seminal plasma to cryopreservation medium can preserve the quality of common carp sperm. The protective effect of each fraction varied, suggesting the presence of distinct components exerting different effects on cryopreserved sperm function.

Název v anglickém jazyce

Seminal plasma fractions can protect common carp (Cyprinus carpio) sperm during cryopreservation

Popis výsledku anglicky

This study aimed to investigate the effect of fractionated seminal plasma on characteristics of common carp Cyprinus carpio cryopreserved sperm. Nanosep (R) centrifugal devices yielded four seminal plasma fractions with different total protein content ranging in molecular weight from less than 17 to almost 74 kDa. Each protein fraction was added to semen extender medium prior to freezing. Spermatozoon motility characteristics and DNA integrity were analyzed in supplemented and non-supplemented cryopreserved samples. The cryopreservation process strongly affected the swim-up sperm quality. Treatment with fractions 1, 2, 3, and 4 was associated with significantly higher spermatozoon motility rate and curvilinear velocity than seen in extender only, with highest values obtained with fraction 4 (78.21 +/- 2.41% and 168.05 +/- 4.46 mu m/s, respectively). Significantly less DNA damage, expressed as percent tail DNA (12.23 +/- 1.27) and olive tail moment (0.68 +/- 0.12), was recorded in fraction 4. The findings indicated that addition of fractionated seminal plasma to cryopreservation medium can preserve the quality of common carp sperm. The protective effect of each fraction varied, suggesting the presence of distinct components exerting different effects on cryopreserved sperm function.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fish Physiology and Biochemistry

ISSN

0920-1742

e-ISSN

—

Svazek periodika

46

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

1461-1468

Kód UT WoS článku

000534193800001

EID výsledku v databázi Scopus

2-s2.0-85085292306