Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43900934" target="_blank" >RIV/60076658:12520/20:43900934 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.17221/181/2019-VETMED" target="_blank" >https://doi.org/10.17221/181/2019-VETMED</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/181/2019-VETMED" target="_blank" >10.17221/181/2019-VETMED</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus)
Popis výsledku v původním jazyce
In this study, we describe the immunohistochemical characterisation of the thymus, the main lymphoid organ, in sturgeon. The wide range cytokeratin, vimentin, S-100 protein, LCA ( CD45) and CD3 were selected as the immunohistochemical markers to map the thymus in juvenile sterlet (Acipenser ruthenus). The epithelial cells and Hassall's corpuscles were labelled with a wide range cytokeratin. The fibroblasts and connective tissue within the thin fibrous capsule on the thymic surface expressed vimentin positivity. The stromal reticular cells were S-100 protein positive. The Leukocyte Common Antigen LCA (CD45) was negative on the thymic lymphocytes. The CD3 was negative on the thymic lymphocytes with cross-reactivity on the non-targeted structures. In conclusion, the commercially available antibodies against the wide range cytokeratin, vimentin and S-100 protein can be used to differentiate components of the sturgeon thymus, while the LCA (CD45) and CD3 application failed. We suggest that further studies are needed to generate fish specific antibodies.
Název v anglickém jazyce
Immunohistochemical mapping of thymic microenvironment in sterlet (Acipenser ruthenus)
Popis výsledku anglicky
In this study, we describe the immunohistochemical characterisation of the thymus, the main lymphoid organ, in sturgeon. The wide range cytokeratin, vimentin, S-100 protein, LCA ( CD45) and CD3 were selected as the immunohistochemical markers to map the thymus in juvenile sterlet (Acipenser ruthenus). The epithelial cells and Hassall's corpuscles were labelled with a wide range cytokeratin. The fibroblasts and connective tissue within the thin fibrous capsule on the thymic surface expressed vimentin positivity. The stromal reticular cells were S-100 protein positive. The Leukocyte Common Antigen LCA (CD45) was negative on the thymic lymphocytes. The CD3 was negative on the thymic lymphocytes with cross-reactivity on the non-targeted structures. In conclusion, the commercially available antibodies against the wide range cytokeratin, vimentin and S-100 protein can be used to differentiate components of the sturgeon thymus, while the LCA (CD45) and CD3 application failed. We suggest that further studies are needed to generate fish specific antibodies.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Veterinární medicína
ISSN
0375-8427
e-ISSN
—
Svazek periodika
65
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
8
Strana od-do
301-308
Kód UT WoS článku
000548361200003
EID výsledku v databázi Scopus
2-s2.0-85090612018