Cryopreservation of Germ Stem Cells in Fish

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901834" target="_blank" >RIV/60076658:12520/20:43901834 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cryopreservation of Germ Stem Cells in Fish

Popis výsledku v původním jazyce

Germ cells have drawn much attention as gamete precursors because of their exceptional potential for fundamental biological studies, as well as for being a potential alternative to gene resource banking for breeding and restocking purposes via surrogacy. Research has developed markers for germ cell identification, shedding light on their origin and specification mechanism. Then, pioneering studies focused on germ cell transplantation into the surrogate host with donor-derived gamete production have followed suit. The advent of transgenic technologies allowed the development of lines expressing green fluorescent protein (GFP) in their germ cells exclusively or the use of artificially synthesised mRNA conjugated with GFP to label and trace germ cells in vivo and facilitate studies on transplantation. The power of germ cell manipulation techniques was significantly enhanced by the development of a wide range of cryopreservation methods; with these approaches, valuable genetic material can be cryopreserved even during the blastula stage, as well as harvested from an adult specimen. In turn, the germ cells in the cryogenic storage can be recovered by transplantation in the surrogate host, producing donor-derived gametes after maturation. The aim of this chapter is to provide basic knowledge about a relatively novel biotechnological approach in fish reproduction and summarise performed studies on germ cell cryopreservation.

Název v anglickém jazyce

Cryopreservation of Germ Stem Cells in Fish

Popis výsledku anglicky

Germ cells have drawn much attention as gamete precursors because of their exceptional potential for fundamental biological studies, as well as for being a potential alternative to gene resource banking for breeding and restocking purposes via surrogacy. Research has developed markers for germ cell identification, shedding light on their origin and specification mechanism. Then, pioneering studies focused on germ cell transplantation into the surrogate host with donor-derived gamete production have followed suit. The advent of transgenic technologies allowed the development of lines expressing green fluorescent protein (GFP) in their germ cells exclusively or the use of artificially synthesised mRNA conjugated with GFP to label and trace germ cells in vivo and facilitate studies on transplantation. The power of germ cell manipulation techniques was significantly enhanced by the development of a wide range of cryopreservation methods; with these approaches, valuable genetic material can be cryopreserved even during the blastula stage, as well as harvested from an adult specimen. In turn, the germ cells in the cryogenic storage can be recovered by transplantation in the surrogate host, producing donor-derived gametes after maturation. The aim of this chapter is to provide basic knowledge about a relatively novel biotechnological approach in fish reproduction and summarise performed studies on germ cell cryopreservation.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Cryopreservation of Fish Gametes

ISBN

978-981-15-4024-0

Počet stran výsledku

28

Strana od-do

285-312

Počet stran knihy

352

Název nakladatele

Springer Nature Singapore Pte Ltd.

Místo vydání

Singapore

Kód UT WoS kapitoly

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