Specificity of Germ Cell Technologies in Sturgeons

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F20%3A43901835" target="_blank" >RIV/60076658:12520/20:43901835 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Specificity of Germ Cell Technologies in Sturgeons

Popis výsledku v původním jazyce

Sturgeons are one of the oldest, biggest, most valuable and today also most endangered group of fish species. Germ stem cells (GSCs), such us embryonic primordial germ cells (PGCs) or spermatogonial/oogonial stem cells, can be a key for an effective conservation and possible restoration of these unique and astonishing fishes. In this chapter, labeling, development, isolation, and transplantation of GSCs were studied in sturgeons. It was shown that the maternally supplied germ plasm, which determines the PGC origin, is localized in vegetal pole of ovulated egg and remains there throughout the cleavage period, and therefore, the PGC specification pattern is similar to that of anuran amphibians rather than teleostean fishes. This knowledge enabled to develop an original PGC labeling method using common cell tracer dye injection into the vegetal pole of two- to eight-cell stage embryo. Next inhibition of maternally supplied dead end RNA resulted in PGC mismigration and general sterilization of individuals. This method enables preparation of recipients for germ cell transplantation. Isolation and transplantation of spermatogonia and oogonia were developed as well. It was tested that one sturgeon juvenile (Siberian sturgeon) can provide approximately one million germ cells suitable for transplantation. Moreover, it was shown that these cells are capable of propagation via an in vitro culture system and of cryopreservation. After freezing/thawing of sturgeon gonadal tissue followed by enzymatic dissociation, above 90% of viable cells were obtained and used for transplantation. The technique of surrogate production can be applied for conservation and possibly restoration of critically endangered sturgeon species with a long term of maturation and a big body size (e.g., beluga), whereas a more common species with shorter term of maturation and smaller body size (e.g., sterlet) can be used as a recipient (surrogate parent).

Název v anglickém jazyce

Specificity of Germ Cell Technologies in Sturgeons

Popis výsledku anglicky

Sturgeons are one of the oldest, biggest, most valuable and today also most endangered group of fish species. Germ stem cells (GSCs), such us embryonic primordial germ cells (PGCs) or spermatogonial/oogonial stem cells, can be a key for an effective conservation and possible restoration of these unique and astonishing fishes. In this chapter, labeling, development, isolation, and transplantation of GSCs were studied in sturgeons. It was shown that the maternally supplied germ plasm, which determines the PGC origin, is localized in vegetal pole of ovulated egg and remains there throughout the cleavage period, and therefore, the PGC specification pattern is similar to that of anuran amphibians rather than teleostean fishes. This knowledge enabled to develop an original PGC labeling method using common cell tracer dye injection into the vegetal pole of two- to eight-cell stage embryo. Next inhibition of maternally supplied dead end RNA resulted in PGC mismigration and general sterilization of individuals. This method enables preparation of recipients for germ cell transplantation. Isolation and transplantation of spermatogonia and oogonia were developed as well. It was tested that one sturgeon juvenile (Siberian sturgeon) can provide approximately one million germ cells suitable for transplantation. Moreover, it was shown that these cells are capable of propagation via an in vitro culture system and of cryopreservation. After freezing/thawing of sturgeon gonadal tissue followed by enzymatic dissociation, above 90% of viable cells were obtained and used for transplantation. The technique of surrogate production can be applied for conservation and possibly restoration of critically endangered sturgeon species with a long term of maturation and a big body size (e.g., beluga), whereas a more common species with shorter term of maturation and smaller body size (e.g., sterlet) can be used as a recipient (surrogate parent).

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Reproduction in Aquatic Animals

ISBN

978-981-15-2289-5

Počet stran výsledku

22

Strana od-do

335-356

Počet stran knihy

379

Název nakladatele

Springer Nature Singapore Pte Ltd.

Místo vydání

Singapore

Kód UT WoS kapitoly

—