Multiple sperm collection as an effective solution for gamete management in pikeperch (Sander lucioperca)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902485" target="_blank" >RIV/60076658:12520/21:43902485 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aquaculture.2020.735870" target="_blank" >https://doi.org/10.1016/j.aquaculture.2020.735870</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2020.735870" target="_blank" >10.1016/j.aquaculture.2020.735870</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiple sperm collection as an effective solution for gamete management in pikeperch (Sander lucioperca)

Popis výsledku v původním jazyce

The aims of this study were to investigate (a) the effects of single or multiple sperm collection with catheter, and (b) impacts of multiple sampling method (catheter or syringe) on sperm quality in pikeperch (Sander lucioperca). To compare the effects of sampling method, sperm was collected repeatedly from the same fish using catheter (n = 6) or syringe (n = 5) at 48, 52 and 56 h post-hormonal injection. For the single collection group as control, sperm was collected with catheter from 12 fish at 48 h post-injection. The results showed that multiple sperm collection with catheter does not significantly (P&gt; 0.05) influence sperm volume and density compared to single sperm collection. However, multiple collection with syringe led to drastic reduction of sperm density at 56 h post-injection. Moreover, sperm volume was influenced by post-injection time where the highest volume was obtained at 48 h post-injection. Furthermore, sperm density and osmolality of seminal plasma were significantly affected by sampling method and post-injection time where higher values were found for the samples collected with catheter at 48 h post-injection. Number of males producing milt with motile spermatozoa decreased along with multiple sperm collection. Sperm motility at 60 s post-activation was affected by the interaction of sampling method and post-injection time, and the highest value was obtained for the sperm collected with syringe at 48 h post-injection. Sperm curvilinear velocity (VCL) at 15 and 45 s post-activation was affected by sampling method and post-injection time, respectively. These findings contribute to the pikeperch hatchery practices, as multiple sperm collection could be applied during artificial reproduction if limited number of males are available.

Název v anglickém jazyce

Multiple sperm collection as an effective solution for gamete management in pikeperch (Sander lucioperca)

Popis výsledku anglicky

The aims of this study were to investigate (a) the effects of single or multiple sperm collection with catheter, and (b) impacts of multiple sampling method (catheter or syringe) on sperm quality in pikeperch (Sander lucioperca). To compare the effects of sampling method, sperm was collected repeatedly from the same fish using catheter (n = 6) or syringe (n = 5) at 48, 52 and 56 h post-hormonal injection. For the single collection group as control, sperm was collected with catheter from 12 fish at 48 h post-injection. The results showed that multiple sperm collection with catheter does not significantly (P&gt; 0.05) influence sperm volume and density compared to single sperm collection. However, multiple collection with syringe led to drastic reduction of sperm density at 56 h post-injection. Moreover, sperm volume was influenced by post-injection time where the highest volume was obtained at 48 h post-injection. Furthermore, sperm density and osmolality of seminal plasma were significantly affected by sampling method and post-injection time where higher values were found for the samples collected with catheter at 48 h post-injection. Number of males producing milt with motile spermatozoa decreased along with multiple sperm collection. Sperm motility at 60 s post-activation was affected by the interaction of sampling method and post-injection time, and the highest value was obtained for the sperm collected with syringe at 48 h post-injection. Sperm curvilinear velocity (VCL) at 15 and 45 s post-activation was affected by sampling method and post-injection time, respectively. These findings contribute to the pikeperch hatchery practices, as multiple sperm collection could be applied during artificial reproduction if limited number of males are available.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

—

Svazek periodika

530

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

—

Kód UT WoS článku

000582169700020

EID výsledku v databázi Scopus

2-s2.0-85089946527