Brief communication: rapid elimination of egg stickiness using sodium hypochlorite in wels catfish Silurus glanis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902520" target="_blank" >RIV/60076658:12520/21:43902520 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s10695-020-00843-3" target="_blank" >https://doi.org/10.1007/s10695-020-00843-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10695-020-00843-3" target="_blank" >10.1007/s10695-020-00843-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Brief communication: rapid elimination of egg stickiness using sodium hypochlorite in wels catfish Silurus glanis

Popis výsledku v původním jazyce

The wels catfishSilurus glanisis valuable fish for aquaculture. Its production relies mainly on artificial reproduction. One of the crucial steps determining success of the reproduction is elimination of egg stickiness after fertilization. To date, the catfish egg de-adhesion is usually carried out using proteolytic enzymes. Here, we prove a novel method based on oxidation of the egg surface by means of sodium hypochlorite. An effect of different exposure times and concentrations on the egg adhesiveness and damage was tested in the first trial. The selected concentration of sodium hypochlorite 0.3 mg center dot l(-1)with exposure time 40 s was used for comparison with the conventical de-adhesion method using alcalase treatment. The fertilization and hatching rates reached very satisfactory outcome in both treatments (98.3 +/- 0.7% vs 97.5 +/- 2.2% and 86.6 +/- 8.3% vs 91.3 +/- 8.5% in alcalase- and sodium hypochlorite-treated embryos, respectively) without any statistical differences. Thus, the de-adhesion method using sodium hypochlorite can be recommended as a suitable method for wels catfish eggs. The method is simple, cheap, very fast, and the treated eggs are disinfected.

Název v anglickém jazyce

Brief communication: rapid elimination of egg stickiness using sodium hypochlorite in wels catfish Silurus glanis

Popis výsledku anglicky

The wels catfishSilurus glanisis valuable fish for aquaculture. Its production relies mainly on artificial reproduction. One of the crucial steps determining success of the reproduction is elimination of egg stickiness after fertilization. To date, the catfish egg de-adhesion is usually carried out using proteolytic enzymes. Here, we prove a novel method based on oxidation of the egg surface by means of sodium hypochlorite. An effect of different exposure times and concentrations on the egg adhesiveness and damage was tested in the first trial. The selected concentration of sodium hypochlorite 0.3 mg center dot l(-1)with exposure time 40 s was used for comparison with the conventical de-adhesion method using alcalase treatment. The fertilization and hatching rates reached very satisfactory outcome in both treatments (98.3 +/- 0.7% vs 97.5 +/- 2.2% and 86.6 +/- 8.3% vs 91.3 +/- 8.5% in alcalase- and sodium hypochlorite-treated embryos, respectively) without any statistical differences. Thus, the de-adhesion method using sodium hypochlorite can be recommended as a suitable method for wels catfish eggs. The method is simple, cheap, very fast, and the treated eggs are disinfected.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fish Physiology and Biochemistry

ISSN

0920-1742

e-ISSN

—

Svazek periodika

47

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

681-685

Kód UT WoS článku

000548150500001

EID výsledku v databázi Scopus

2-s2.0-85087843700