Identification of QTL linked to heat tolerance in rice (Oryza sativa L.) using SSR markers through bulked segregant analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902557" target="_blank" >RIV/60076658:12520/21:43902557 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.37992/2021.1201.007" target="_blank" >https://doi.org/10.37992/2021.1201.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37992/2021.1201.007" target="_blank" >10.37992/2021.1201.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of QTL linked to heat tolerance in rice (Oryza sativa L.) using SSR markers through bulked segregant analysis

Popis výsledku v původním jazyce

Identification of loci and linked markers enable the marker-assisted selection for transferring the reproductive phase heat tolerance in rice. Microsatellite marker-based bulked segregant analysis (BSA) for heat tolerance was performed in F3 progenies of the cross between temperature sensitive high yielding variety Uma and tolerant variety N22. High-temperature stress ranged from 23.33 to 38.99 °C at the booting-stage, and spikelet-fertility was considered as a direct measure of tolerance. N22 has exhibited a high spikelet-fertility of 90.92 per cent against 2.62 per cent, 44.78 per cent in Uma and F3 plants, respectively. Of the 197 microsatellite markers screened, 41 were found to be polymorphic and used for BSA of tolerant and susceptible F3 bulk along with both the parents. Marker RM5749 on chromosome 4 had co-segregated with tolerance, whereas, previously reported markers for heat tolerance in rice did not have an effect in the study population. The LOD value obtained during single marker analysis for the linkage between marker RM5749 and spikelet fertility was 6.86, indicating a strong linkage with the spikelet fertility under heat stress. The failure of the reported markers in our population suggests that the reproductive phase heat tolerance mechanisms in rice vary across the populations and the markers have to be validated for each population before being employed in MAS. © 2020

Název v anglickém jazyce

Identification of QTL linked to heat tolerance in rice (Oryza sativa L.) using SSR markers through bulked segregant analysis

Popis výsledku anglicky

Identification of loci and linked markers enable the marker-assisted selection for transferring the reproductive phase heat tolerance in rice. Microsatellite marker-based bulked segregant analysis (BSA) for heat tolerance was performed in F3 progenies of the cross between temperature sensitive high yielding variety Uma and tolerant variety N22. High-temperature stress ranged from 23.33 to 38.99 °C at the booting-stage, and spikelet-fertility was considered as a direct measure of tolerance. N22 has exhibited a high spikelet-fertility of 90.92 per cent against 2.62 per cent, 44.78 per cent in Uma and F3 plants, respectively. Of the 197 microsatellite markers screened, 41 were found to be polymorphic and used for BSA of tolerant and susceptible F3 bulk along with both the parents. Marker RM5749 on chromosome 4 had co-segregated with tolerance, whereas, previously reported markers for heat tolerance in rice did not have an effect in the study population. The LOD value obtained during single marker analysis for the linkage between marker RM5749 and spikelet fertility was 6.86, indicating a strong linkage with the spikelet fertility under heat stress. The failure of the reported markers in our population suggests that the reproductive phase heat tolerance mechanisms in rice vary across the populations and the markers have to be validated for each population before being employed in MAS. © 2020

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electronic Journal of Plant Breeding

ISSN

0975-928X

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

8

Strana od-do

46-53

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85104268460