The stochastic association of nanoparticles with algae at the cellular level: Effects of NOM, particle size and particle shape

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902625" target="_blank" >RIV/60076658:12520/21:43902625 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.ecoenv.2021.112280" target="_blank" >https://doi.org/10.1016/j.ecoenv.2021.112280</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ecoenv.2021.112280" target="_blank" >10.1016/j.ecoenv.2021.112280</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The stochastic association of nanoparticles with algae at the cellular level: Effects of NOM, particle size and particle shape

Popis výsledku v původním jazyce

Association of nanoparticles (NPs) with algae likely plays a critical role in their transfer in aquatic food chains. Although our understanding of the ecotoxicity and fate of NPs in the environment is increasing, it is still unclear how the physicochemical properties of NPs influence their interaction with algae at cellular levels and how this is reflected at a population level. This is due to the limitation in the existing analytical techniques to quantify the association of NPs with cells. To fill this data gap, we applied the novel technique of single-cell inductively coupled plasma mass spectrometry to quantify the cellular association of gold (Au)-NPs with algal cells (Pseudokirchneriella subcapitata) as a function of particle size, shape (spherical 10 nm, spherical 60 nm, spherical 100 nm, rod-shaped 10 x 40 nm, and rod-shaped 50 x 100 nm), and surface chemistry [citrate and natural organic matter (NOM) coating] on a cell-by-cell basis. The association of Au-NPs with algal cells was found to be a random probability following a so-called stochastic process; after 72 h of exposure, less than 45% of the cell population accumulated NPs on their surface. The number of Au-NPs per cell was found to be heterogeneously distributed as some cells were associated with a significantly higher number (e.g. up to 600 spherical 10 nm particles per cell) of Au-NPs than other cells present in the medium. The presence of NOM on the surface of the particles decreased the percentage of cells containing NPs except for the spherical 60 nm Au-NPs. We conclude that some algae within a population can accumulate NPs on their surface and this accumulation is influenced by the size, shape, and surface chemistry of NPs. It is important to understand how NPs may enter aquatic food chains to assess the possible risk.

Název v anglickém jazyce

The stochastic association of nanoparticles with algae at the cellular level: Effects of NOM, particle size and particle shape

Popis výsledku anglicky

Association of nanoparticles (NPs) with algae likely plays a critical role in their transfer in aquatic food chains. Although our understanding of the ecotoxicity and fate of NPs in the environment is increasing, it is still unclear how the physicochemical properties of NPs influence their interaction with algae at cellular levels and how this is reflected at a population level. This is due to the limitation in the existing analytical techniques to quantify the association of NPs with cells. To fill this data gap, we applied the novel technique of single-cell inductively coupled plasma mass spectrometry to quantify the cellular association of gold (Au)-NPs with algal cells (Pseudokirchneriella subcapitata) as a function of particle size, shape (spherical 10 nm, spherical 60 nm, spherical 100 nm, rod-shaped 10 x 40 nm, and rod-shaped 50 x 100 nm), and surface chemistry [citrate and natural organic matter (NOM) coating] on a cell-by-cell basis. The association of Au-NPs with algal cells was found to be a random probability following a so-called stochastic process; after 72 h of exposure, less than 45% of the cell population accumulated NPs on their surface. The number of Au-NPs per cell was found to be heterogeneously distributed as some cells were associated with a significantly higher number (e.g. up to 600 spherical 10 nm particles per cell) of Au-NPs than other cells present in the medium. The presence of NOM on the surface of the particles decreased the percentage of cells containing NPs except for the spherical 60 nm Au-NPs. We conclude that some algae within a population can accumulate NPs on their surface and this accumulation is influenced by the size, shape, and surface chemistry of NPs. It is important to understand how NPs may enter aquatic food chains to assess the possible risk.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10620 - Other biological topics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Ecotoxicology and Environmental Safety

ISSN

0147-6513

e-ISSN

—

Svazek periodika

218

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

—

Kód UT WoS článku

000663326500005

EID výsledku v databázi Scopus

2-s2.0-85105246385