Using fish spermatozoa in in vitro toxicity tests: A potential toxicology tool

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902630" target="_blank" >RIV/60076658:12520/21:43902630 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aquaculture.2021.736647" target="_blank" >https://doi.org/10.1016/j.aquaculture.2021.736647</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aquaculture.2021.736647" target="_blank" >10.1016/j.aquaculture.2021.736647</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Using fish spermatozoa in in vitro toxicity tests: A potential toxicology tool

Popis výsledku v původním jazyce

In recent years, the development and implementation of new in vitro techniques for ecotoxicology and environmental risk assessment has become a priority; however, the current data indicate a lack of standardization in available in vitro sperm tests. In this review, we describe the main approaches currently used for in vitro toxicity testing of fish spermatozoa. Though fish species show huge diversity in their reproductive strategies, most are external fertilizers. Fish spermatozoa are highly specialized, transcriptionally inactive cells that become motile only upon spawning in the aqueous environment. Here, we suggest that they could become a useful toxicology tool because of their simplicity and availability. Indeed, fish spermatozoa are a promising model for genotoxicity testing, since parental exposure to genotoxicants, both in vivo and in vitro, affect embryo development. In aquaculture practice it means that evaluation of spermatozoa DNA damage could help in predicting embryo quality. Fish spermatozoa could also be useful for the study on nongenomic mechanisms of toxicity, such as the effects of xenobiotics on protein posttranslational modifications. Nevertheless, we conclude that further studies on fish spermatozoa are required to establish a clear correlation between in vitro and in vivo toxicities.

Název v anglickém jazyce

Using fish spermatozoa in in vitro toxicity tests: A potential toxicology tool

Popis výsledku anglicky

In recent years, the development and implementation of new in vitro techniques for ecotoxicology and environmental risk assessment has become a priority; however, the current data indicate a lack of standardization in available in vitro sperm tests. In this review, we describe the main approaches currently used for in vitro toxicity testing of fish spermatozoa. Though fish species show huge diversity in their reproductive strategies, most are external fertilizers. Fish spermatozoa are highly specialized, transcriptionally inactive cells that become motile only upon spawning in the aqueous environment. Here, we suggest that they could become a useful toxicology tool because of their simplicity and availability. Indeed, fish spermatozoa are a promising model for genotoxicity testing, since parental exposure to genotoxicants, both in vivo and in vitro, affect embryo development. In aquaculture practice it means that evaluation of spermatozoa DNA damage could help in predicting embryo quality. Fish spermatozoa could also be useful for the study on nongenomic mechanisms of toxicity, such as the effects of xenobiotics on protein posttranslational modifications. Nevertheless, we conclude that further studies on fish spermatozoa are required to establish a clear correlation between in vitro and in vivo toxicities.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Aquaculture

ISSN

0044-8486

e-ISSN

—

Svazek periodika

539

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

—

Kód UT WoS článku

000647594200008

EID výsledku v databázi Scopus

2-s2.0-85103328129