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Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F21%3A43902836" target="_blank" >RIV/60076658:12520/21:43902836 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://doi.org/10.1016/j.aquaculture.2021.737113" target="_blank" >https://doi.org/10.1016/j.aquaculture.2021.737113</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.aquaculture.2021.737113" target="_blank" >10.1016/j.aquaculture.2021.737113</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet

  • Popis výsledku v původním jazyce

    This study examined the effect of konjac glucomannan (KGM) supplementation in diets with different lipid levels on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in juvenile pompano (Trachinotus ovatus). Triplicate groups of fish (12.07 +/- 0.21 g) were fed eight experimental diets containing two lipid levels of 9% (basal-fat diet, BFD) and 18% (high-fat diet, HFD), and four levels of KGM (0, 0.5, 1 and 2%) for 60 days. Pompano fed HFD exhibited markedly lower growth and feed efficiency than fish fed BFD, and KGM supplementation, particularly at 1% inclusion, significantly improved growth performance and feed efficiency in fish fed both BFD and HFD. In addition, Pompano fed HFD showed higher whole-body lipid and lower protein contents than fish fed BFD. Irrespective of dietary lipid level, whole body, dorsal and ventral muscle were reduced by dietary KGM supplementation up to 1% while the opposite was found for whole body protein content. Dietary KGM also significantly reduced the HSI, liver lipid contents, as well as lipid droplets in the liver. Supplementation of KGM to both BFD and HFD significantly reduced serum total cholesterol, triglyceride, lowdensity lipoprotein cholesterol and free fatty acids levels. Furthermore, irrespective of dietary lipid level, increasing dietary KGM supplementation enhanced serum IgM level, lysozyme and alkaline phosphatase activities, and reduced serum alanine aminotransferase and aspartate aminotransferase activities. Dietary KGM also increased liver total antioxidant capacity, superoxide dismutase and catalase activities, and decreased malondialdehyde concentration in fish fed both HFD and BFD. Dietary KGM significantly increased serum glutathione peroxidase, superoxide dismutase and catalase activities and reduced malondialdehyde concentrations in fish fed BFD, and enhanced serum total antioxidant capacity and glutathione peroxidase activity in fish fed HFD. Supplementation of KGM increased the expression of genes associated with lipid catabolism (ppar alpha, cpt1, hsl) and lipid transport (fabp1, apoB100 and cd36), and decreased the expression of lipid anabolism related genes (srebp1, ppar gamma, fas and acc). Moreover, the expression of pro-inflammatory cytokines including tumor necrosis factor alpha and interleukin-8 were down-regulated, while expression of the anti-inflammatory cytokine transforming growth factor beta 1 was increased by KGM supplementation. In conclusion, supplementation of KGM at 1% of diet improved growth, feed efficiency, antioxidant capacity, hepatic lipid metabolism and inflammatory responses in juvenile pompano, and can mitigate excessive deposition of lipid in tissues of farmed fish.

  • Název v anglickém jazyce

    Impacts of dietary konjac glucomannan supplementation on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in golden pompano (Trachinotus ovatus) fed a high fat diet

  • Popis výsledku anglicky

    This study examined the effect of konjac glucomannan (KGM) supplementation in diets with different lipid levels on growth, antioxidant capacity, hepatic lipid metabolism and inflammatory response in juvenile pompano (Trachinotus ovatus). Triplicate groups of fish (12.07 +/- 0.21 g) were fed eight experimental diets containing two lipid levels of 9% (basal-fat diet, BFD) and 18% (high-fat diet, HFD), and four levels of KGM (0, 0.5, 1 and 2%) for 60 days. Pompano fed HFD exhibited markedly lower growth and feed efficiency than fish fed BFD, and KGM supplementation, particularly at 1% inclusion, significantly improved growth performance and feed efficiency in fish fed both BFD and HFD. In addition, Pompano fed HFD showed higher whole-body lipid and lower protein contents than fish fed BFD. Irrespective of dietary lipid level, whole body, dorsal and ventral muscle were reduced by dietary KGM supplementation up to 1% while the opposite was found for whole body protein content. Dietary KGM also significantly reduced the HSI, liver lipid contents, as well as lipid droplets in the liver. Supplementation of KGM to both BFD and HFD significantly reduced serum total cholesterol, triglyceride, lowdensity lipoprotein cholesterol and free fatty acids levels. Furthermore, irrespective of dietary lipid level, increasing dietary KGM supplementation enhanced serum IgM level, lysozyme and alkaline phosphatase activities, and reduced serum alanine aminotransferase and aspartate aminotransferase activities. Dietary KGM also increased liver total antioxidant capacity, superoxide dismutase and catalase activities, and decreased malondialdehyde concentration in fish fed both HFD and BFD. Dietary KGM significantly increased serum glutathione peroxidase, superoxide dismutase and catalase activities and reduced malondialdehyde concentrations in fish fed BFD, and enhanced serum total antioxidant capacity and glutathione peroxidase activity in fish fed HFD. Supplementation of KGM increased the expression of genes associated with lipid catabolism (ppar alpha, cpt1, hsl) and lipid transport (fabp1, apoB100 and cd36), and decreased the expression of lipid anabolism related genes (srebp1, ppar gamma, fas and acc). Moreover, the expression of pro-inflammatory cytokines including tumor necrosis factor alpha and interleukin-8 were down-regulated, while expression of the anti-inflammatory cytokine transforming growth factor beta 1 was increased by KGM supplementation. In conclusion, supplementation of KGM at 1% of diet improved growth, feed efficiency, antioxidant capacity, hepatic lipid metabolism and inflammatory responses in juvenile pompano, and can mitigate excessive deposition of lipid in tissues of farmed fish.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    40103 - Fishery

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2021

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Aquaculture

  • ISSN

    0044-8486

  • e-ISSN

  • Svazek periodika

    545

  • Číslo periodika v rámci svazku

    neuveden

  • Stát vydavatele periodika

    NL - Nizozemsko

  • Počet stran výsledku

    14

  • Strana od-do

  • Kód UT WoS článku

    000692122600009

  • EID výsledku v databázi Scopus

    2-s2.0-85111614255