Evaluation of absorbent cotton for glycopeptide enrichment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43905241" target="_blank" >RIV/60076658:12520/22:43905241 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s00216-022-04353-4" target="_blank" >https://doi.org/10.1007/s00216-022-04353-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-022-04353-4" target="_blank" >10.1007/s00216-022-04353-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of absorbent cotton for glycopeptide enrichment

Popis výsledku v původním jazyce

Selecting proper and efficient glycopeptide enrichment approaches are essential for mass spectrometry-based glycoproteomics since glycopeptides are usually with microheterogeneity and low abundance in most biological samples. Herein, we introduced a cotton hydrophilic interaction liquid chromatography (HILIC) approach for large-scale glycopeptide enrichment with 80% acetonitrile/1% trifluoroacetic acid as the optimal sample loading buffer. The comparison of cotton HILIC with Venusil HILIC and mixed anion-exchange (MAX) approaches indicated that cotton HILIC was superior in overall glycopeptide enrichment, whereas Venusil HILIC preferred in complex glycan structures and MAX performed better with high mannose glycans. Exploration of capacity and recovery rate of cotton HILIC illustrated that 5mg cotton packed in a 200 mu L tip achieved a reasonable glycopeptide enrichment performance (similar to 6% recovery) from similar to 0.5mg peptides. In conclusion, cotton HILIC can be used as an optional glycopeptide enrichment approach in glycosylation analysis with its specific merit.

Název v anglickém jazyce

Evaluation of absorbent cotton for glycopeptide enrichment

Popis výsledku anglicky

Selecting proper and efficient glycopeptide enrichment approaches are essential for mass spectrometry-based glycoproteomics since glycopeptides are usually with microheterogeneity and low abundance in most biological samples. Herein, we introduced a cotton hydrophilic interaction liquid chromatography (HILIC) approach for large-scale glycopeptide enrichment with 80% acetonitrile/1% trifluoroacetic acid as the optimal sample loading buffer. The comparison of cotton HILIC with Venusil HILIC and mixed anion-exchange (MAX) approaches indicated that cotton HILIC was superior in overall glycopeptide enrichment, whereas Venusil HILIC preferred in complex glycan structures and MAX performed better with high mannose glycans. Exploration of capacity and recovery rate of cotton HILIC illustrated that 5mg cotton packed in a 200 mu L tip achieved a reasonable glycopeptide enrichment performance (similar to 6% recovery) from similar to 0.5mg peptides. In conclusion, cotton HILIC can be used as an optional glycopeptide enrichment approach in glycosylation analysis with its specific merit.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

1618-2650

Svazek periodika

414

Číslo periodika v rámci svazku

29-30

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

8245-8253

Kód UT WoS článku

000862528800004

EID výsledku v databázi Scopus

2-s2.0-85139235783