Evaluation of establishment and maintenance of primary cell cultures from several strains of common carp (Cyprinus carpio L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F22%3A43905244" target="_blank" >RIV/60076658:12520/22:43905244 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1111/jfb.15232" target="_blank" >https://doi.org/10.1111/jfb.15232</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jfb.15232" target="_blank" >10.1111/jfb.15232</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of establishment and maintenance of primary cell cultures from several strains of common carp (Cyprinus carpio L.)

Popis výsledku v původním jazyce

As a surrogate for the whole organism, primary cultures and cell lines serve as valuable tools for investigating exogenous and endogenous cytopathy. Studying cell responsiveness to diseases and contaminants is considered a less demanding and more readily accessible research approach that minimizes animal distress and provides more specific data. In the current work, the authors established primary cultures from several different organs and tissues of common carp (Cyprinus carpio L.) for subsequent use in other applications. They investigated the technical challenges in obtaining successful and durable carp-derived tissue cultures. The trials indicate that the type of tissue grown, carp strain and fish age impact equally upon culturing success, as do the cultivating conditions. Cells from gill epithelia, head and trunk kidneys, spleen, skin, gonads and ocular tissue were successfully established and maintained for further use in in-vitro testing. The primary cultures were, therefore, used to investigate and assess pathogens and pollutants emerging in carp&apos;s environment.

Název v anglickém jazyce

Evaluation of establishment and maintenance of primary cell cultures from several strains of common carp (Cyprinus carpio L.)

Popis výsledku anglicky

As a surrogate for the whole organism, primary cultures and cell lines serve as valuable tools for investigating exogenous and endogenous cytopathy. Studying cell responsiveness to diseases and contaminants is considered a less demanding and more readily accessible research approach that minimizes animal distress and provides more specific data. In the current work, the authors established primary cultures from several different organs and tissues of common carp (Cyprinus carpio L.) for subsequent use in other applications. They investigated the technical challenges in obtaining successful and durable carp-derived tissue cultures. The trials indicate that the type of tissue grown, carp strain and fish age impact equally upon culturing success, as do the cultivating conditions. Cells from gill epithelia, head and trunk kidneys, spleen, skin, gonads and ocular tissue were successfully established and maintained for further use in in-vitro testing. The primary cultures were, therefore, used to investigate and assess pathogens and pollutants emerging in carp&apos;s environment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Fish Biology

ISSN

0022-1112

e-ISSN

—

Svazek periodika

101

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1634-1643

Kód UT WoS článku

000869325600001

EID výsledku v databázi Scopus

2-s2.0-85139930499