Species-specific differences in DNA damage sensitivity at early developmental stage: A comparative study of sterlet ( Acipenser ruthenus ) and common carp ( Cyprinus carpio )

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F24%3A43908192" target="_blank" >RIV/60076658:12520/24:43908192 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.etap.2024.104501" target="_blank" >https://doi.org/10.1016/j.etap.2024.104501</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.etap.2024.104501" target="_blank" >10.1016/j.etap.2024.104501</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Species-specific differences in DNA damage sensitivity at early developmental stage: A comparative study of sterlet ( Acipenser ruthenus ) and common carp ( Cyprinus carpio )

Popis výsledku v původním jazyce

DNA damage in embryos shapes the development of an organism. Understanding life stage-specific differences between fish species is essential for ecological risk assessment measures. We explored DNA damage sensitivity in two nonmodel fish species, sterlet (Acipenser ruthenus) and common carp (Cyprinus carpio). Embryos of these species were exposed to a model genotoxicant, camptothecin (CPT), during cleavage (2-cell) stage and gastrulation. Results revealed a species-specific DNA damage sensitivity only at cleavage stage. 3 nM CPT caused lethality in sterlet embryos while carp embryos hatched normally. Multiple nuclear abnormalities were observed in sterlet embryos by early gastrula stage. However, carp embryos exhibited nuclear abnormalities and DNA fragmentation at neurula stage only when exposed to 7 nM CPT. Moreover, increased expression of tp53 in carp embryos at gastrula stage suggests activation of apoptosis mechanism. These findings suggest that carp embryos activate DNA damage response more efficiently than sterlet embryos at same developmental stage.

Název v anglickém jazyce

Species-specific differences in DNA damage sensitivity at early developmental stage: A comparative study of sterlet ( Acipenser ruthenus ) and common carp ( Cyprinus carpio )

Popis výsledku anglicky

DNA damage in embryos shapes the development of an organism. Understanding life stage-specific differences between fish species is essential for ecological risk assessment measures. We explored DNA damage sensitivity in two nonmodel fish species, sterlet (Acipenser ruthenus) and common carp (Cyprinus carpio). Embryos of these species were exposed to a model genotoxicant, camptothecin (CPT), during cleavage (2-cell) stage and gastrulation. Results revealed a species-specific DNA damage sensitivity only at cleavage stage. 3 nM CPT caused lethality in sterlet embryos while carp embryos hatched normally. Multiple nuclear abnormalities were observed in sterlet embryos by early gastrula stage. However, carp embryos exhibited nuclear abnormalities and DNA fragmentation at neurula stage only when exposed to 7 nM CPT. Moreover, increased expression of tp53 in carp embryos at gastrula stage suggests activation of apoptosis mechanism. These findings suggest that carp embryos activate DNA damage response more efficiently than sterlet embryos at same developmental stage.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10617 - Marine biology, freshwater biology, limnology

Projekt

<a href="/cs/project/EF16_025%2F0007370" target="_blank" >EF16_025/0007370: Reprodukční a genetické postupy pro uchování biodiverzity ryb a akvakulturu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Toxicology and Pharmacology

ISSN

1382-6689

e-ISSN

1872-7077

Svazek periodika

110

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

001280854700001

EID výsledku v databázi Scopus

2-s2.0-85199352810