Effects of mifepristone, a model compound with anti-progestogenic activity, on the reproduction of African clawed frog ( Xenopus laevis)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F24%3A43908411" target="_blank" >RIV/60076658:12520/24:43908411 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aquatox.2023.106694" target="_blank" >https://doi.org/10.1016/j.aquatox.2023.106694</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jhazmat.2024.136493" target="_blank" >10.1016/j.jhazmat.2024.136493</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of mifepristone, a model compound with anti-progestogenic activity, on the reproduction of African clawed frog ( Xenopus laevis)

Popis výsledku v původním jazyce

This is the first study on how a substance with anti-progestogenic activity affects amphibian reproduction. Mifepristone, a synthetic anti-progestin used in abortion pills, was chosen as model compound. African clawed frog ( Xenopus laevis) females were exposed to four mifepristone concentrations (0.7, 9, 120, and 1380 ng center dot L-1) for 30 days. A control group was also included. Egg-laying during the experiment was significantly less at the highest concentration. At the experiment&apos;s end, mifepristone-exposed and control females were randomly mated with sexually mature males. Breeding rate for females exposed to 1380 ng center dot L-1 mifepristone was only 50 %. Histology revealed no significant changes in gonads, thyroid, or liver. Females exposed to 1380 ng center dot L-1 mifepristone had lower estradiol levels in plasma, lower mRNA expression of lh and fsh in brain-pituitary complex, and p450scc in ovaries. In liver, mRNA level of npr was significantly increased in females exposed to 120 ng center dot L-1 mifepristone. mRNA expression of mpr, er beta, dio2, and dio3 were upregulated in animals exposed to 9 ng center dot L-1 and 120 ng center dot L-1 mifepristone, whereas vtg expression was significantly downregulated in females exposed to 1380 ng center dot L-1 mifepristone. All these findings show that exposure to compounds with anti-progestogenic activity affects the hypothalamus-pituitary-gonad axis and decreases reproductive success.

Název v anglickém jazyce

Effects of mifepristone, a model compound with anti-progestogenic activity, on the reproduction of African clawed frog ( Xenopus laevis)

Popis výsledku anglicky

This is the first study on how a substance with anti-progestogenic activity affects amphibian reproduction. Mifepristone, a synthetic anti-progestin used in abortion pills, was chosen as model compound. African clawed frog ( Xenopus laevis) females were exposed to four mifepristone concentrations (0.7, 9, 120, and 1380 ng center dot L-1) for 30 days. A control group was also included. Egg-laying during the experiment was significantly less at the highest concentration. At the experiment&apos;s end, mifepristone-exposed and control females were randomly mated with sexually mature males. Breeding rate for females exposed to 1380 ng center dot L-1 mifepristone was only 50 %. Histology revealed no significant changes in gonads, thyroid, or liver. Females exposed to 1380 ng center dot L-1 mifepristone had lower estradiol levels in plasma, lower mRNA expression of lh and fsh in brain-pituitary complex, and p450scc in ovaries. In liver, mRNA level of npr was significantly increased in females exposed to 120 ng center dot L-1 mifepristone. mRNA expression of mpr, er beta, dio2, and dio3 were upregulated in animals exposed to 9 ng center dot L-1 and 120 ng center dot L-1 mifepristone, whereas vtg expression was significantly downregulated in females exposed to 1380 ng center dot L-1 mifepristone. All these findings show that exposure to compounds with anti-progestogenic activity affects the hypothalamus-pituitary-gonad axis and decreases reproductive success.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

<a href="/cs/project/GA22-19136S" target="_blank" >GA22-19136S: Charakterizace účinků látek s anti-progestagenní aktivitou vyskytujících se ve vodním prostředí na nižší obratlovce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Hazardous Materials

ISSN

0304-3894

e-ISSN

1873-3336

Svazek periodika

480

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

001359132300001

EID výsledku v databázi Scopus

2-s2.0-85209116492