Dietary tauroursodeoxycholic acid supports growth and gut health in spotted seabass (Lateolabrax maculatus) under thermal stress conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909700" target="_blank" >RIV/60076658:12520/25:43909700 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.fsi.2025.110423" target="_blank" >https://doi.org/10.1016/j.fsi.2025.110423</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fsi.2025.110423" target="_blank" >10.1016/j.fsi.2025.110423</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dietary tauroursodeoxycholic acid supports growth and gut health in spotted seabass (Lateolabrax maculatus) under thermal stress conditions

Popis výsledku v původním jazyce

An 8-week feeding experiment was carries out to explore the impacts of dietary tauroursodeoxycholic acid (TUDCA) on growth and gut integrity in spotted seabass (Lateolabrax maculatus) under heat stress (33 degrees C). Three hundred fish (2 +/- 0.02 g) were allocated to triplicate groups and fed five diets containing graded levels of TUDCA at 0, 10, 20, 30, or 40 mg/kg (designated as Con, T10, T20, T30 and T40 diets). Growth performance was significantly (P &lt; 0.05) enhanced in fish receiving &gt;= 30 mg/kg TUDCA compared to the control group. Progressive increases in intestinal total antioxidant capacity and superoxide dismutase activity, accompanied by decreased malondialdehyde concentration, were observed as TUDCA dose increased. TUDCA application modulated the expression of intestinal antioxidant-related genes, downregulating keap1 and upregulating nrf2. Notably, supplementation with 40 mg/kg TUDCA improved intestinal morphology, as evidenced by increased villus height and number. Furthermore, in the T40 group, a marked downregulation of pro-apoptotic genes (caspase3, caspase8, caspase9, and bax) and reduced immunofluorescence intensity were observed, while the expression of the anti-apoptotic gene bcl was significantly up-regulated. Additionally, the expression of pro-inflammatory genes (il-1 beta, il-8, and tnf-alpha) and immunofluorescence intensity were significantly reduced in the T40 group compared to control. In contrast, the expression of anti-inflammatory genes (il-4, il-10, and tgf-beta) was markedly upregulated. Furthermore, dietary inclusion of 40 mg/kg TUDCA suppressed the expression of endoplasmic reticulum stress-related genes (grp78, chop, perk, atf6, and ire1) and activated the bile acid receptor gene tgr5 in the intestine. Concurrently, TUDCA treatment enhanced the PI3K-Akt signaling pathway, contributing to the inhibition of apoptosis. The data generated in this study demonstrated that dietary supplementation with 40 mg/kg TUDCA promotes growth, activates the Nrf2-Keap1 and PI3K-AKT signaling pathways, enhances intestinal antioxidant defenses, suppresses inflammation and apoptosis, alleviates endoplasmic reticulum stress, and mitigates the physiological impacts of heat stress in L. maculatus reared at elevated temperatures.

Název v anglickém jazyce

Dietary tauroursodeoxycholic acid supports growth and gut health in spotted seabass (Lateolabrax maculatus) under thermal stress conditions

Popis výsledku anglicky

An 8-week feeding experiment was carries out to explore the impacts of dietary tauroursodeoxycholic acid (TUDCA) on growth and gut integrity in spotted seabass (Lateolabrax maculatus) under heat stress (33 degrees C). Three hundred fish (2 +/- 0.02 g) were allocated to triplicate groups and fed five diets containing graded levels of TUDCA at 0, 10, 20, 30, or 40 mg/kg (designated as Con, T10, T20, T30 and T40 diets). Growth performance was significantly (P &lt; 0.05) enhanced in fish receiving &gt;= 30 mg/kg TUDCA compared to the control group. Progressive increases in intestinal total antioxidant capacity and superoxide dismutase activity, accompanied by decreased malondialdehyde concentration, were observed as TUDCA dose increased. TUDCA application modulated the expression of intestinal antioxidant-related genes, downregulating keap1 and upregulating nrf2. Notably, supplementation with 40 mg/kg TUDCA improved intestinal morphology, as evidenced by increased villus height and number. Furthermore, in the T40 group, a marked downregulation of pro-apoptotic genes (caspase3, caspase8, caspase9, and bax) and reduced immunofluorescence intensity were observed, while the expression of the anti-apoptotic gene bcl was significantly up-regulated. Additionally, the expression of pro-inflammatory genes (il-1 beta, il-8, and tnf-alpha) and immunofluorescence intensity were significantly reduced in the T40 group compared to control. In contrast, the expression of anti-inflammatory genes (il-4, il-10, and tgf-beta) was markedly upregulated. Furthermore, dietary inclusion of 40 mg/kg TUDCA suppressed the expression of endoplasmic reticulum stress-related genes (grp78, chop, perk, atf6, and ire1) and activated the bile acid receptor gene tgr5 in the intestine. Concurrently, TUDCA treatment enhanced the PI3K-Akt signaling pathway, contributing to the inhibition of apoptosis. The data generated in this study demonstrated that dietary supplementation with 40 mg/kg TUDCA promotes growth, activates the Nrf2-Keap1 and PI3K-AKT signaling pathways, enhances intestinal antioxidant defenses, suppresses inflammation and apoptosis, alleviates endoplasmic reticulum stress, and mitigates the physiological impacts of heat stress in L. maculatus reared at elevated temperatures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fish &amp; Shellfish Immunology

ISSN

1050-4648

e-ISSN

1095-9947

Svazek periodika

163

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

nestránkováno

Kód UT WoS článku

001498298800001

EID výsledku v databázi Scopus

2-s2.0-105005216958