Effect of reduced osmolality in glucose-based extenders on the post-thaw motility of Mediterranean brown trout (Salmo cettii) spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43909841" target="_blank" >RIV/60076658:12520/25:43909841 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3389/fmars.2025.1621496" target="_blank" >https://doi.org/10.3389/fmars.2025.1621496</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmars.2025.1621496" target="_blank" >10.3389/fmars.2025.1621496</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of reduced osmolality in glucose-based extenders on the post-thaw motility of Mediterranean brown trout (Salmo cettii) spermatozoa

Popis výsledku v původním jazyce

The current study aimed to compare the effects of decreasing osmolality in glucose-based extenders containing methanol alone versus a methanol-ethylene glycol combination on the post-thaw motility of Mediterranean brown trout (Salmo cettii) spermatozoa. Milt was collected from mature males in the Biferno River, Southern Italy, and cryopreserved using a control cryomedium (150 mM glucose with 7.5% methanol) and experimental variants with reduced glucose (100 mM, 50 mM) paired with methanol alone or combined with 2.5% ethylene glycol. Samples were cryopreserved in 0.25 mL straws with a final sperm concentration of 3.0 x 10(9) spermatozoa/mL, corresponding to 750 x 10(6) spermatozoa/straw. Sperm motility and kinematic parameters of Mediterranean brown trout were assessed for fresh sperm and post-thaw samples cryopreserved in various cryomedia. Fresh sperm exhibited robust motility (89.0 +/- 8.4%) and curvilinear velocity (VCL, 121.2 +/- 22.4 mu m/s), while the control cryomedium preserved motility at 65.9 +/- 12.9% and VCL at 117.6 +/- 26.1 mu m/s; the experimental 100 mM glucose with 7.5% methanol and 2.5% ethylene glycol treatment yielded comparable motility (65.6 +/- 11.6%) but reduced VCL (81.5 +/- 16.1 mu m/s, p &lt; 0.05). Other treatments showed diminished efficacy, particularly at 50 mM glucose. These findings reveal that a hypotonic extender with methanol-ethylene glycol synergy sustains motility after cryopreservation despite lower glucose levels, demonstrating that the osmotic threshold of extender can be lowered to 100 mOsm/kg without compromising salmonid sperm function. This approach offers a practical tool for conserving S. cettii, supporting broader restoration efforts amid escalating environmental pressures.

Název v anglickém jazyce

Effect of reduced osmolality in glucose-based extenders on the post-thaw motility of Mediterranean brown trout (Salmo cettii) spermatozoa

Popis výsledku anglicky

The current study aimed to compare the effects of decreasing osmolality in glucose-based extenders containing methanol alone versus a methanol-ethylene glycol combination on the post-thaw motility of Mediterranean brown trout (Salmo cettii) spermatozoa. Milt was collected from mature males in the Biferno River, Southern Italy, and cryopreserved using a control cryomedium (150 mM glucose with 7.5% methanol) and experimental variants with reduced glucose (100 mM, 50 mM) paired with methanol alone or combined with 2.5% ethylene glycol. Samples were cryopreserved in 0.25 mL straws with a final sperm concentration of 3.0 x 10(9) spermatozoa/mL, corresponding to 750 x 10(6) spermatozoa/straw. Sperm motility and kinematic parameters of Mediterranean brown trout were assessed for fresh sperm and post-thaw samples cryopreserved in various cryomedia. Fresh sperm exhibited robust motility (89.0 +/- 8.4%) and curvilinear velocity (VCL, 121.2 +/- 22.4 mu m/s), while the control cryomedium preserved motility at 65.9 +/- 12.9% and VCL at 117.6 +/- 26.1 mu m/s; the experimental 100 mM glucose with 7.5% methanol and 2.5% ethylene glycol treatment yielded comparable motility (65.6 +/- 11.6%) but reduced VCL (81.5 +/- 16.1 mu m/s, p &lt; 0.05). Other treatments showed diminished efficacy, particularly at 50 mM glucose. These findings reveal that a hypotonic extender with methanol-ethylene glycol synergy sustains motility after cryopreservation despite lower glucose levels, demonstrating that the osmotic threshold of extender can be lowered to 100 mOsm/kg without compromising salmonid sperm function. This approach offers a practical tool for conserving S. cettii, supporting broader restoration efforts amid escalating environmental pressures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Marine Science

ISSN

2296-7745

e-ISSN

2296-7745

Svazek periodika

12

Číslo periodika v rámci svazku

neuvedeno

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

7

Strana od-do

nestránkováno

Kód UT WoS článku

001549653900001

EID výsledku v databázi Scopus

2-s2.0-105012953963