Gentamicin preserves motility and viability of Zebrafish (Danio rerio) sperm inoculated with bacteria during short-term storage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F25%3A43910051" target="_blank" >RIV/60076658:12520/25:43910051 - isvavai.cz</a>

Výsledek na webu

<a href="http://jifro.ir/article-1-5695-fa.html" target="_blank" >http://jifro.ir/article-1-5695-fa.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.22092/ijfs.2025.133805" target="_blank" >10.22092/ijfs.2025.133805</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Gentamicin preserves motility and viability of Zebrafish (Danio rerio) sperm inoculated with bacteria during short-term storage

Popis výsledku v původním jazyce

Sperm short-term storage at low temperatures provides an advantage to control artificial reproduction in fish. However, spermatozoa lose their potential for motility and fertilization during the storage period that may be associated with microbial growth. In the present study, we used Zebrafish (Danio rerio) sperm to investigate the effects of bacterial contamination on spermatozoa motility and viability during short-term storage. Spermatozoa retained better motility characteristics during the storage period when sperm was diluted with an extender compared to the undiluted sperm. Inoculation of sperm with bacteria decreased spermatozoa motility in a concentration-dependent manner. Through the storage period, spermatozoa motility was not affected in sperm inoculated with 103 CFU/mL Vibrio harveyi or Pseudomonas aeruginosa. A significant decrease in spermatozoa motility was observed in the sperm inoculated with 108 CFU/mL V. harveyi and with 105 CFU/mL P. aeruginosa at 3 h post-storage. Next, we determined the effective concentrations of gentamicin to preserve spermatozoa against bacteria during short-term storage. The spermatozoa motility was similar to that of non-infected sperm when 0.08-0.15 and 0.02-0.04 mg/mL gentamicin was added to the sperm inoculated with 108 CFU/mL V. harveyi or P. aeruginosa, respectively. At 24 h post-storage, spermatozoa viability was significantly decreased in the sperm inoculated with 108 CFU/mL V. harveyi or P. aeruginosa, while it showed an increasing trend of spermatozoa viability in bacterially infected sperm with gentamicin. The results showed the detrimental effects of bacteria on spermatozoa motility during short-term storage, and determined effective dose of gentamicin to preserve spermatozoa against V. harveyi or P. aeruginosa. This study provides valuable information to develop artificial in Zebrafish research centers.

Název v anglickém jazyce

Gentamicin preserves motility and viability of Zebrafish (Danio rerio) sperm inoculated with bacteria during short-term storage

Popis výsledku anglicky

Sperm short-term storage at low temperatures provides an advantage to control artificial reproduction in fish. However, spermatozoa lose their potential for motility and fertilization during the storage period that may be associated with microbial growth. In the present study, we used Zebrafish (Danio rerio) sperm to investigate the effects of bacterial contamination on spermatozoa motility and viability during short-term storage. Spermatozoa retained better motility characteristics during the storage period when sperm was diluted with an extender compared to the undiluted sperm. Inoculation of sperm with bacteria decreased spermatozoa motility in a concentration-dependent manner. Through the storage period, spermatozoa motility was not affected in sperm inoculated with 103 CFU/mL Vibrio harveyi or Pseudomonas aeruginosa. A significant decrease in spermatozoa motility was observed in the sperm inoculated with 108 CFU/mL V. harveyi and with 105 CFU/mL P. aeruginosa at 3 h post-storage. Next, we determined the effective concentrations of gentamicin to preserve spermatozoa against bacteria during short-term storage. The spermatozoa motility was similar to that of non-infected sperm when 0.08-0.15 and 0.02-0.04 mg/mL gentamicin was added to the sperm inoculated with 108 CFU/mL V. harveyi or P. aeruginosa, respectively. At 24 h post-storage, spermatozoa viability was significantly decreased in the sperm inoculated with 108 CFU/mL V. harveyi or P. aeruginosa, while it showed an increasing trend of spermatozoa viability in bacterially infected sperm with gentamicin. The results showed the detrimental effects of bacteria on spermatozoa motility during short-term storage, and determined effective dose of gentamicin to preserve spermatozoa against V. harveyi or P. aeruginosa. This study provides valuable information to develop artificial in Zebrafish research centers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40103 - Fishery

Projekt

<a href="/cs/project/QK21010141" target="_blank" >QK21010141: Management gamet a reprodukce při ochraně genetických zdrojů a šlechtitelském programu kapra obecného v podmínkách akvakultury ČR</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Iranian Journal of Fisheries Sciences

ISSN

1562-2916

e-ISSN

—

Svazek periodika

24

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

IR - Íránská islámská republika

Počet stran výsledku

17

Strana od-do

633-649

Kód UT WoS článku

001524077500012

EID výsledku v databázi Scopus

2-s2.0-105006693924