Specifická aktivita fosfatázy vázané na povrch buněk u různých morfotypů bakterioplanktonu v acidifikovaném horském jezeře

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F06%3A00042946" target="_blank" >RIV/60077344:_____/06:00042946 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/06:00008770

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake

Popis výsledku v původním jazyce

Activity of extracellular acid phosphatases was measured at single-cell level in bacterioplankton groups defined by their morphology and size, in acidified mountain Lake Čertovo, using the substrate ELF97 phosphate. Fluorescent precipitates resulting from the substrate hydrolysis by phosphatases were quantified by image analysis. A conspicuous morphotype of curved cells, despite its low total biomass (average of 4%), contributed significantly (in average by 31%) to the total bacterioplankton phosphataseactivity. Bacterial filaments, although comprising in average 85% of bacterioplankton biomass, contributed to the total bacterioplankton activity only by 45%. Biomass-specific activity of extracellular (cell-surface) phosphatases of the main bacterioplankton morphotypes increased in the order: filaments < cocci and rods < curved cells. The biomass-specific activity of bacterioplankton extracellular phosphatases was generally highest in the spring and decreased gradually during summer.

Název v anglickém jazyce

Specific activity of cell-surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake

Popis výsledku anglicky

Activity of extracellular acid phosphatases was measured at single-cell level in bacterioplankton groups defined by their morphology and size, in acidified mountain Lake Čertovo, using the substrate ELF97 phosphate. Fluorescent precipitates resulting from the substrate hydrolysis by phosphatases were quantified by image analysis. A conspicuous morphotype of curved cells, despite its low total biomass (average of 4%), contributed significantly (in average by 31%) to the total bacterioplankton phosphataseactivity. Bacterial filaments, although comprising in average 85% of bacterioplankton biomass, contributed to the total bacterioplankton activity only by 45%. Biomass-specific activity of extracellular (cell-surface) phosphatases of the main bacterioplankton morphotypes increased in the order: filaments < cocci and rods < curved cells. The biomass-specific activity of bacterioplankton extracellular phosphatases was generally highest in the spring and decreased gradually during summer.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA6017202" target="_blank" >IAA6017202: Detekce, lokalizace a kvantifikace extracelulárních enzymatických aktivit na buněčné a populační úrovni v přirozeném sestonu stojatých vod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Microbiology

ISSN

1462-2912

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

1271-1279

Kód UT WoS článku

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EID výsledku v databázi Scopus

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