Izolace a charakterizace vysoce repetitivní frakce genomu banánovníku

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F07%3A00305799" target="_blank" >RIV/60077344:_____/07:00305799 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/07:00305799

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation and characterization of the highly repeated fraction of the banana genome

Popis výsledku v původním jazyce

Although the nuclear genome of banana (Musa spp.) is relatively small (1C 610 Mbp for M. acuminata ), the results obtained from other sequenced genomes suggest that more than half of the banana genome may be composed of repetitive and non-coding DNA sequences. Knowledge of repetitive DNA can facilitate mapping of important traits, phylogenetic studies, BAC-based physical mapping, and genome sequencing/annotation. However, only a few repetitive DNA sequences have been characterized in banana. In this work, we used DNA reassociation kinetics to isolate the highly repeated fraction of the banana genome (M. acuminata Calcutta 4?). Two libraries, one prepared from Cot < 0.05 DNA (2,688 clones) and one from Cot < 0.1 sequences (4,608 clones), were constructed, and 614 DNA clones were chosen randomly for sequencing and further characterization. Dot-plot analysis revealed that 14% of the sequenced clones contained various semi-tandem and palindromic repeated sequences. BLAST? homology searches

Název v anglickém jazyce

Isolation and characterization of the highly repeated fraction of the banana genome

Popis výsledku anglicky

Although the nuclear genome of banana (Musa spp.) is relatively small (1C 610 Mbp for M. acuminata ), the results obtained from other sequenced genomes suggest that more than half of the banana genome may be composed of repetitive and non-coding DNA sequences. Knowledge of repetitive DNA can facilitate mapping of important traits, phylogenetic studies, BAC-based physical mapping, and genome sequencing/annotation. However, only a few repetitive DNA sequences have been characterized in banana. In this work, we used DNA reassociation kinetics to isolate the highly repeated fraction of the banana genome (M. acuminata Calcutta 4?). Two libraries, one prepared from Cot < 0.05 DNA (2,688 clones) and one from Cot < 0.1 sequences (4,608 clones), were constructed, and 614 DNA clones were chosen randomly for sequencing and further characterization. Dot-plot analysis revealed that 14% of the sequenced clones contained various semi-tandem and palindromic repeated sequences. BLAST? homology searches

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cytogenetics and Genome Research

ISSN

1424-8581

e-ISSN

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Svazek periodika

119

Číslo periodika v rámci svazku

3-4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

7

Strana od-do

268-274

Kód UT WoS článku

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EID výsledku v databázi Scopus

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