Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00331350" target="_blank" >RIV/60077344:_____/09:00331350 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio

Popis výsledku v původním jazyce

We examined the composition of telomeres of parasitic worms, representatives of flatworm groups Monogenea and Cestoda Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala), by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomere repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, Nippotaenia mogurndae) but not in Acanthocephala Pomphorhynchus laevis and P. tereticollis). In the Pomphorhynchus species, no hybridisation signals were obtained either with the nematode? (TTAGGC)n, arthropod? (TTAGG)n and bdelloid (TGTGGG)n, telomeric probes using the FISH with their chromosomes and Southern hybridization with P. laevis DNA. Therefore, we suggest that parasitic Acanthocephala have evolved yet unknown telomeric repeat motif or different mechanism of telomere maintenance.

Název v anglickém jazyce

Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio

Popis výsledku anglicky

We examined the composition of telomeres of parasitic worms, representatives of flatworm groups Monogenea and Cestoda Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala), by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomere repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, Nippotaenia mogurndae) but not in Acanthocephala Pomphorhynchus laevis and P. tereticollis). In the Pomphorhynchus species, no hybridisation signals were obtained either with the nematode? (TTAGGC)n, arthropod? (TTAGG)n and bdelloid (TGTGGG)n, telomeric probes using the FISH with their chromosomes and Southern hybridization with P. laevis DNA. Therefore, we suggest that parasitic Acanthocephala have evolved yet unknown telomeric repeat motif or different mechanism of telomere maintenance.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA206%2F06%2F1860" target="_blank" >GA206/06/1860: Molekulární evoluce pohlavních chromosomů motýlů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genome

ISSN

0831-2796

e-ISSN

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Svazek periodika

52

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CA - Kanada

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000272206700001

EID výsledku v databázi Scopus

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