Kinetoplastid guide RNA biogenesis is dependent on subunits of the mitochondrial RNA binding complex 1 and mitochondrial RNA polymerase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00334909" target="_blank" >RIV/60077344:_____/09:00334909 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/09:00010578

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Kinetoplastid guide RNA biogenesis is dependent on subunits of the mitochondrial RNA binding complex 1 and mitochondrial RNA polymerase

Popis výsledku v původním jazyce

The mitochondrial RNA binding complex 1 (MRB1) is a recently discovered set of proteins associated with the TbRGG1 in Trypanosoma brucei. Based on the phenotype caused by downregulation of this protein, it was proposed to play an unspecified RNA editingrole. RNAi-silencing of three newly characterized protein subunits, guide RNA associated proteins (GAPs) 1 and 2 plus a predicted RNA helicase, show they are essential for cell growth in the insect stage. Furthermore, their downregulation leads to inhibition of editing in only those mRNAs for which minicircle-encoded guide (g) RNAs are required. All three proteins are necessary for the expression of the minicircle-encoded gRNAs. Moreover, down-regulation of a fourth assayed putative MRB1 subunit, Nudixhydrolase, does not appear to destabilize gRNAs but has a general impact on the stability of maxicircle-encoded RNAs. We also demonstrate that the same mt RNA polymerase may also have a role in expression of the minicircle-encoded gRNAs.

Název v anglickém jazyce

Kinetoplastid guide RNA biogenesis is dependent on subunits of the mitochondrial RNA binding complex 1 and mitochondrial RNA polymerase

Popis výsledku anglicky

The mitochondrial RNA binding complex 1 (MRB1) is a recently discovered set of proteins associated with the TbRGG1 in Trypanosoma brucei. Based on the phenotype caused by downregulation of this protein, it was proposed to play an unspecified RNA editingrole. RNAi-silencing of three newly characterized protein subunits, guide RNA associated proteins (GAPs) 1 and 2 plus a predicted RNA helicase, show they are essential for cell growth in the insect stage. Furthermore, their downregulation leads to inhibition of editing in only those mRNAs for which minicircle-encoded guide (g) RNAs are required. All three proteins are necessary for the expression of the minicircle-encoded gRNAs. Moreover, down-regulation of a fourth assayed putative MRB1 subunit, Nudixhydrolase, does not appear to destabilize gRNAs but has a general impact on the stability of maxicircle-encoded RNAs. We also demonstrate that the same mt RNA polymerase may also have a role in expression of the minicircle-encoded gRNAs.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

R N A

ISSN

1355-8382

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

00264280100008

EID výsledku v databázi Scopus

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