Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00334916" target="_blank" >RIV/60077344:_____/09:00334916 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12220/09:00010834

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

Popis výsledku v původním jazyce

A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial SSU rRNA and GP60 partial genes as well as PCR-RFLP of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and 2 sows (10%). Twenty one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and C. suis in finishers were found. Both sows were infected with the C. parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.

Název v anglickém jazyce

Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

Popis výsledku anglicky

A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial SSU rRNA and GP60 partial genes as well as PCR-RFLP of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and 2 sows (10%). Twenty one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and C. suis in finishers were found. Both sows were infected with the C. parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/GP523%2F07%2FP117" target="_blank" >GP523/07/P117: Zoonotický potenciál kryptosporidií infikujících skot a prasata</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Parasitology Research

ISSN

0932-0113

e-ISSN

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Svazek periodika

104

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

000262652600031

EID výsledku v databázi Scopus

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