Chlorophyll biosynthesis and chloroplast development in etiolated seedlings of Ginkgo biloba L

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F09%3A00352587" target="_blank" >RIV/60077344:_____/09:00352587 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Chlorophyll biosynthesis and chloroplast development in etiolated seedlings of Ginkgo biloba L

Popis výsledku v původním jazyce

Ginkgo biloba is a large tree native in China with evolutionary affinities to the conifers and cycads. However unlike conifers, the gymnosperm G.biloba is not able to synthesize chlorophyll (Chl) in the dark, in spite of the presence of genes encoding subunits of light-independent protochlorophyllide oxidoreductase (DPOR) in the plastid genome. The principal aims of the present study were to investigate the presence of DPOR protein subunits as well as the key regulatory step in Chl formation: aminolevulinic acid synthesis and abundance of the key regulatory enzyme in its synthesis: glutamyl-tRNA reductase. In addition, functional stage of photosynthetic apparatus and assembly of pigment-protein complexes were investigated. Dark-grown, illuminated and circadian-grown seedlings were used. Our results clearly showed that no protein subunits of DPOR were detected irrespective of light conditions, what is consistent with the absence of Chl and Chl-binding proteins in the dark.

Název v anglickém jazyce

Chlorophyll biosynthesis and chloroplast development in etiolated seedlings of Ginkgo biloba L

Popis výsledku anglicky

Ginkgo biloba is a large tree native in China with evolutionary affinities to the conifers and cycads. However unlike conifers, the gymnosperm G.biloba is not able to synthesize chlorophyll (Chl) in the dark, in spite of the presence of genes encoding subunits of light-independent protochlorophyllide oxidoreductase (DPOR) in the plastid genome. The principal aims of the present study were to investigate the presence of DPOR protein subunits as well as the key regulatory step in Chl formation: aminolevulinic acid synthesis and abundance of the key regulatory enzyme in its synthesis: glutamyl-tRNA reductase. In addition, functional stage of photosynthetic apparatus and assembly of pigment-protein complexes were investigated. Dark-grown, illuminated and circadian-grown seedlings were used. Our results clearly showed that no protein subunits of DPOR were detected irrespective of light conditions, what is consistent with the absence of Chl and Chl-binding proteins in the dark.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Photosynthetica

ISSN

0300-3604

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000275416900004

EID výsledku v databázi Scopus

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