Toward the crystallization of photosystem II core complex from Pisum sativum L

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00355837" target="_blank" >RIV/60077344:_____/10:00355837 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/10:00355837 RIV/68378050:_____/10:00355837 RIV/60076658:12640/10:00012204

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Toward the crystallization of photosystem II core complex from Pisum sativum L

Popis výsledku v původním jazyce

The crystallization of the higher plant similar to 1 gigadalton homodimeric lipid-pigment-protein complex of photosystem II constitutes a challenging undertaking that has not been crowned with success to date. In the continuation of our previous efforts(Kuta-Smatanova et al. Photosyn. Res. 2006, 90, 255-259) that had provided needle and plate crystals of plant photosystem II for the first time, we report on a study to crystallize three-dimensional crystals of the photosystem 11 from Pisum sativum L. suitable for X-ray analysis. In our screening, we optimized temperature, supersaturation rate, and concentrations of detergents, buffers, salts, and organic additives. We used a sitting and hanging drop vapor-diffusion method, microbatch under oil and counter-diffusion techniques, and direct and cross-influenced procedures. We have identified that the major limiting factors for the growth of crystals are the photosystem II stability and requirement of indispensable divalent cations.

Název v anglickém jazyce

Toward the crystallization of photosystem II core complex from Pisum sativum L

Popis výsledku anglicky

The crystallization of the higher plant similar to 1 gigadalton homodimeric lipid-pigment-protein complex of photosystem II constitutes a challenging undertaking that has not been crowned with success to date. In the continuation of our previous efforts(Kuta-Smatanova et al. Photosyn. Res. 2006, 90, 255-259) that had provided needle and plate crystals of plant photosystem II for the first time, we report on a study to crystallize three-dimensional crystals of the photosystem 11 from Pisum sativum L. suitable for X-ray analysis. In our screening, we optimized temperature, supersaturation rate, and concentrations of detergents, buffers, salts, and organic additives. We used a sitting and hanging drop vapor-diffusion method, microbatch under oil and counter-diffusion techniques, and direct and cross-influenced procedures. We have identified that the major limiting factors for the growth of crystals are the photosystem II stability and requirement of indispensable divalent cations.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06010" target="_blank" >LC06010: Centrum biokatalýzy a biotransformací</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Crystal Growth & Design

ISSN

1528-7483

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000280471700017

EID výsledku v databázi Scopus

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