The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F10%3A00360066" target="_blank" >RIV/60077344:_____/10:00360066 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria

Popis výsledku v původním jazyce

The changes that occurred in the diversity of microbes in soil and compost during its passage through the gut of earthworms were evaluated in two epigeic earthworm species Eisenia fetida (Savigny, 1826) and E. andrei (Bouché, 1972), which inhabit forestsoil and garden organic compost, respectively. A modified extraction protocol was used to extract DNA from forest soil, compost and fresh earthworm faeces. DNA was amplified in a polymerase chain reaction (PCR). The products were analyzed using denaturing gradient gel electrophoresis (DGGE) fingerprinting. Analysis of archaeal and bacterial DGGE fingerprints revealed that the diversity in both microbial domains increased after passage through the guts of E. andrei, but not E. fetida. Nevertheless, cluster and correspondence analyses revealed that the passage of soil and compost through the guts of E. fetida, E. andrei, respectively, had a significant effect on the composition of the archaea.

Název v anglickém jazyce

The effect of passage through the gut of earthworms (Annelida: Lumbricidae) on the diversity of archaea and bacteria

Popis výsledku anglicky

The changes that occurred in the diversity of microbes in soil and compost during its passage through the gut of earthworms were evaluated in two epigeic earthworm species Eisenia fetida (Savigny, 1826) and E. andrei (Bouché, 1972), which inhabit forestsoil and garden organic compost, respectively. A modified extraction protocol was used to extract DNA from forest soil, compost and fresh earthworm faeces. DNA was amplified in a polymerase chain reaction (PCR). The products were analyzed using denaturing gradient gel electrophoresis (DGGE) fingerprinting. Analysis of archaeal and bacterial DGGE fingerprints revealed that the diversity in both microbial domains increased after passage through the guts of E. andrei, but not E. fetida. Nevertheless, cluster and correspondence analyses revealed that the passage of soil and compost through the guts of E. fetida, E. andrei, respectively, had a significant effect on the composition of the archaea.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Societatis Zoologicae Bohemicae

ISSN

1211-376X

e-ISSN

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Svazek periodika

74

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

69-74

Kód UT WoS článku

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EID výsledku v databázi Scopus

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