Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00358401" target="_blank" >RIV/60077344:_____/11:00358401 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/11:43870554

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10709-011-9546-4" target="_blank" >http://dx.doi.org/10.1007/s10709-011-9546-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10709-011-9546-4" target="_blank" >10.1007/s10709-011-9546-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families

Popis výsledku v původním jazyce

We investigated physical genomic features in Russsian wheat aphid (Diuraphis noxia), a worldwide pest, by karyotype analysis using differential staining with AgNO3, CMA3, and DAPI, by chromosomal localization of ribosomal DNA (rDNA), H3 and H4 histone genes, and the telomeric sequence (TTAGG)n using fluorescence in situ hybridization (FISH), and by measuring the RWA genome size using flow cytometry. The female karyotype, 2n = 10, has a pair of X chromosomes, whereas the male karyotype, 2n = 9, has a single X. The X chromosome is the largest element in the karyotype and 18S rRNA. H3 and H4 gene clusters are colocalized at one of its ends. Interestingly, D. noxia lacks the (TTAGG)n telomeric sequence in its genome. The female genome size is 2C = 0.86 pg,whereas the male genome size is 2C = 0.70 pg. The X chromosome occupies about 35% of the female haploid genome (1C = 0.43 pg), which makes it one of the largest sex chromosomes in the animal kingdom.

Název v anglickém jazyce

Karyotype analysis of the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae) reveals a large X chromosome with rRNA and histone gene families

Popis výsledku anglicky

We investigated physical genomic features in Russsian wheat aphid (Diuraphis noxia), a worldwide pest, by karyotype analysis using differential staining with AgNO3, CMA3, and DAPI, by chromosomal localization of ribosomal DNA (rDNA), H3 and H4 histone genes, and the telomeric sequence (TTAGG)n using fluorescence in situ hybridization (FISH), and by measuring the RWA genome size using flow cytometry. The female karyotype, 2n = 10, has a pair of X chromosomes, whereas the male karyotype, 2n = 9, has a single X. The X chromosome is the largest element in the karyotype and 18S rRNA. H3 and H4 gene clusters are colocalized at one of its ends. Interestingly, D. noxia lacks the (TTAGG)n telomeric sequence in its genome. The female genome size is 2C = 0.86 pg,whereas the male genome size is 2C = 0.70 pg. The X chromosome occupies about 35% of the female haploid genome (1C = 0.43 pg), which makes it one of the largest sex chromosomes in the animal kingdom.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genetica

ISSN

0016-6707

e-ISSN

—

Svazek periodika

139

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

281-289

Kód UT WoS článku

000288514100001

EID výsledku v databázi Scopus

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