MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00359407" target="_blank" >RIV/60077344:_____/11:00359407 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/11:43880918

Výsledek na webu

<a href="http://dx.doi.org/10.1261/rna.2446311" target="_blank" >http://dx.doi.org/10.1261/rna.2446311</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1261/rna.2446311" target="_blank" >10.1261/rna.2446311</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process

Popis výsledku v původním jazyce

In this study we examined the function of the mitochondrial RNA-binding complex 1 (MRB1) protein of Trypanosoma brucei, Tb927.5.3010, which we term MRB3010. We show that MRB3010 is essential for growth of both procyclic form and bloodstream form life-cycle stages of T. brucei. Down-regulation of MRB3010 by RNAi leads to a dramatic inhibition of RNA editing, yet its depletion does not impact total gRNA levels. Rather, it appears to affect the editing process at an early stage, as indicated by the accumulation of pre-edited and small partially edited RNAs. MRB3010 is present in large (>20S) complexes and exhibits both RNA-dependent and RNA-independent interactions with other MRB1 complex proteins. Comparison of proteins isolated with MRB3010 tagged at its endogenous locus to those reported from other MRB1 complex purifications strongly suggests the presence of an MRB1 "core'' complex containing five to six proteins, including MRB3010.

Název v anglickém jazyce

MRB3010 is a core component of the MRB1 complex that facilitates an early step of the kinetoplastid RNA editing process

Popis výsledku anglicky

In this study we examined the function of the mitochondrial RNA-binding complex 1 (MRB1) protein of Trypanosoma brucei, Tb927.5.3010, which we term MRB3010. We show that MRB3010 is essential for growth of both procyclic form and bloodstream form life-cycle stages of T. brucei. Down-regulation of MRB3010 by RNAi leads to a dramatic inhibition of RNA editing, yet its depletion does not impact total gRNA levels. Rather, it appears to affect the editing process at an early stage, as indicated by the accumulation of pre-edited and small partially edited RNAs. MRB3010 is present in large (>20S) complexes and exhibits both RNA-dependent and RNA-independent interactions with other MRB1 complex proteins. Comparison of proteins isolated with MRB3010 tagged at its endogenous locus to those reported from other MRB1 complex purifications strongly suggests the presence of an MRB1 "core'' complex containing five to six proteins, including MRB3010.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

R N A

ISSN

1355-8382

e-ISSN

—

Svazek periodika

17

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

865-877

Kód UT WoS článku

000289617400009

EID výsledku v databázi Scopus

—