New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00359446" target="_blank" >RIV/60077344:_____/11:00359446 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/11:43882557 RIV/60076658:12220/11:43882557

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.vetpar.2010.11.010" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2010.11.010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetpar.2010.11.010" target="_blank" >10.1016/j.vetpar.2010.11.010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II

Popis výsledku v původním jazyce

Two species of Cryptosporidium are commonly identified in pigs: C. suis and C. pig genotype II. Detection is routinely based on molecular methods such as PCR and subsequent RFLP or gene sequencing. Low sensitivity to mixed infections is solved by designing and testing novel species-specific primers in the present study. Sensitivity of our primers was identical to genus-specific primers, but more (1:48) mixed infections were detected using these species-specific primers on 477 DNA samples originating from naturally infected pigs of different age categories. Our results show differences in age-dependent susceptibility of pigs to the infection. Whereas C. suis was found in all tested categories of pigs (1-12 week of age and sows). Cryptosporidium pig genotype II was recorded only in animals older than 6 week of age. Usage of species-specific primers could help to better the understanding of epidemiology and occurrence (often underestimated) of pig specific Cryptosporidium spp.

Název v anglickém jazyce

New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II

Popis výsledku anglicky

Two species of Cryptosporidium are commonly identified in pigs: C. suis and C. pig genotype II. Detection is routinely based on molecular methods such as PCR and subsequent RFLP or gene sequencing. Low sensitivity to mixed infections is solved by designing and testing novel species-specific primers in the present study. Sensitivity of our primers was identical to genus-specific primers, but more (1:48) mixed infections were detected using these species-specific primers on 477 DNA samples originating from naturally infected pigs of different age categories. Our results show differences in age-dependent susceptibility of pigs to the infection. Whereas C. suis was found in all tested categories of pigs (1-12 week of age and sows). Cryptosporidium pig genotype II was recorded only in animals older than 6 week of age. Usage of species-specific primers could help to better the understanding of epidemiology and occurrence (often underestimated) of pig specific Cryptosporidium spp.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/GP523%2F07%2FP117" target="_blank" >GP523/07/P117: Zoonotický potenciál kryptosporidií infikujících skot a prasata</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Parasitology

ISSN

0304-4017

e-ISSN

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Svazek periodika

176

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

120-125

Kód UT WoS článku

000289221400004

EID výsledku v databázi Scopus

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