Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00372946" target="_blank" >RIV/60077344:_____/11:00372946 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers

Popis výsledku v původním jazyce

Bacterial canker of tomato, caused by bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) is a well-known disease resulting in serious losses to both greenhouse and field tomato crops in areas where tomatoes are planted. Cmm was detected and isolated from various parts of strongly infected plants of 10 indeterminate tomato cultivars. The most sensitive technique was optimized PCR with Dreier´s and laboratory-generated PCR primers from the Cmm tomatinase gene. All samples taken from base, stemand petiole of tomato plants were positive in PCR with both primer pairs, and also using IIF and PTA-ELISA in contrast to the samples taken from leaves. Using IIF and PTA-ELISA with PAbs of Neogen Europe Ltd. (UK), Cmm was reliably detected to dilution1:1000 and 1:100 respectively. Seeds of 4 indeterminate and 4 determinate cultivars were consecutively artificially infected by the mixture of the two reference Cmm strains (BCCM/LMG 7333 and 5727) soaking 48 hours in inoculum (108 CFU/ml

Název v anglickém jazyce

Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers

Popis výsledku anglicky

Bacterial canker of tomato, caused by bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) is a well-known disease resulting in serious losses to both greenhouse and field tomato crops in areas where tomatoes are planted. Cmm was detected and isolated from various parts of strongly infected plants of 10 indeterminate tomato cultivars. The most sensitive technique was optimized PCR with Dreier´s and laboratory-generated PCR primers from the Cmm tomatinase gene. All samples taken from base, stemand petiole of tomato plants were positive in PCR with both primer pairs, and also using IIF and PTA-ELISA in contrast to the samples taken from leaves. Using IIF and PTA-ELISA with PAbs of Neogen Europe Ltd. (UK), Cmm was reliably detected to dilution1:1000 and 1:100 respectively. Seeds of 4 indeterminate and 4 determinate cultivars were consecutively artificially infected by the mixture of the two reference Cmm strains (BCCM/LMG 7333 and 5727) soaking 48 hours in inoculum (108 CFU/ml

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QH71229" target="_blank" >QH71229: Diagnostika a metody integrované ochrany proti karanténním a dalším ekonomicky významným patogenům plodové a listové zeleniny</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

III International Symposium on Tomato Diseases

ISBN

9789066056145

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

57-60

Název nakladatele

ISHS Acta Horticulturae

Místo vydání

Leuven

Místo konání akce

Ischia, Naples

Datum konání akce

25. 7. 2010

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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