Analysis of recombinant anticancerogenic nuclease RTBN1 in ?leaf factory? system upon co-expression of modified genes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F11%3A00375331" target="_blank" >RIV/60077344:_____/11:00375331 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of recombinant anticancerogenic nuclease RTBN1 in ?leaf factory? system upon co-expression of modified genes

Popis výsledku v původním jazyce

RTBN1, which is a nuclease with antitumor activity, is currently produced for biomedical experiments using ?leaf factory system. To study stability of its expression, several potential plant expression modificators were cloned and co-expressed with nuclease using A. tumefaciens infiltration system. The nuclease analyses revealed that nuclease TBN1 is quite stable in the used ?leaf factory system as to its molecular mass and glycosylation pattern, only some quantitative changes of nuclease activity weredetected. Our experiments showed that activity and production of the nucleases increased significantly upon co-infiltration with silencing suppressor p19 and decreased dramatically upon co-expression with morphogenesis changing transcription factor HlMyb3. Selected glycosyltransferases and kinases influenced activity and production only insignificantly.

Název v anglickém jazyce

Analysis of recombinant anticancerogenic nuclease RTBN1 in ?leaf factory? system upon co-expression of modified genes

Popis výsledku anglicky

RTBN1, which is a nuclease with antitumor activity, is currently produced for biomedical experiments using ?leaf factory system. To study stability of its expression, several potential plant expression modificators were cloned and co-expressed with nuclease using A. tumefaciens infiltration system. The nuclease analyses revealed that nuclease TBN1 is quite stable in the used ?leaf factory system as to its molecular mass and glycosylation pattern, only some quantitative changes of nuclease activity weredetected. Our experiments showed that activity and production of the nucleases increased significantly upon co-infiltration with silencing suppressor p19 and decreased dramatically upon co-expression with morphogenesis changing transcription factor HlMyb3. Selected glycosyltransferases and kinases influenced activity and production only insignificantly.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA521%2F06%2F1149" target="_blank" >GA521/06/1149: Molekulární analýza homologů rostlinných nukleáz a jejich protinádorový potenciál ve srovnání s živočišnými ribonukleázami</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of abstracts

ISBN

978-80-8105-265-1

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

316-319

Název nakladatele

Univerzita svätého Cyrila a Metoda v Trnave

Místo vydání

Trnava

Místo konání akce

Častá-Papiernička

Datum konání akce

5. 10. 2011

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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