Wolbachia and DNA barcoding Insects: Patterns, potential, and problems

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00378011" target="_blank" >RIV/60077344:_____/12:00378011 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/12:43883570

Výsledek na webu

<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036514" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0036514</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0036514" target="_blank" >10.1371/journal.pone.0036514</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Wolbachia and DNA barcoding Insects: Patterns, potential, and problems

Popis výsledku v původním jazyce

Wolbachia is a genus of bacterial endosymbionts that impacts the breeding systems of their hosts and can confuse the patterns of mitochondrial variation, including DNA barcodes. We examined the extent to which is Wolbachia detected in DNA barcoding, assessed its impact upon identification accuracy, and considered the variation present in Wolbachia COI. When two million insect COI trace files were examined on the Barcode of Life Datasystem, Wolbachia COI was present in 0.16% of the cases. It is possibleto generate Wolbachia COI using standard insect primers, but that amplicon was never confused with host COI. Wolbachia alleles recovered were predominantly Supergroup A and were broadly distributed geographically and phylogenetically. The presence of theWolbachia DNA in total genomic extracts made from insects is unlikely to compromise the accuracy of the DNA barcode library. Regular assays for Wolbachia presence and type should be adopted by large scale insect barcoding initiatives.

Název v anglickém jazyce

Wolbachia and DNA barcoding Insects: Patterns, potential, and problems

Popis výsledku anglicky

Wolbachia is a genus of bacterial endosymbionts that impacts the breeding systems of their hosts and can confuse the patterns of mitochondrial variation, including DNA barcodes. We examined the extent to which is Wolbachia detected in DNA barcoding, assessed its impact upon identification accuracy, and considered the variation present in Wolbachia COI. When two million insect COI trace files were examined on the Barcode of Life Datasystem, Wolbachia COI was present in 0.16% of the cases. It is possibleto generate Wolbachia COI using standard insect primers, but that amplicon was never confused with host COI. Wolbachia alleles recovered were predominantly Supergroup A and were broadly distributed geographically and phylogenetically. The presence of theWolbachia DNA in total genomic extracts made from insects is unlikely to compromise the accuracy of the DNA barcode library. Regular assays for Wolbachia presence and type should be adopted by large scale insect barcoding initiatives.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP505%2F10%2F0673" target="_blank" >GAP505/10/0673: Mezidruhové vztahy ve složitých potravních sítích tropických deštných lesů: experimenální manipulace celého společenstva</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000305341500082

EID výsledku v databázi Scopus

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