Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00380582" target="_blank" >RIV/60077344:_____/12:00380582 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/12:00380582

Výsledek na webu

<a href="http://home.ueb.cas.cz/publikace/2012_Navratil_IOBC_61-67.pdf" target="_blank" >http://home.ueb.cas.cz/publikace/2012_Navratil_IOBC_61-67.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack

Popis výsledku v původním jazyce

Extracellular serine proteases are often used as a factor of pathogenicity. To improve potato resistance to the pathogens, we prepared plants containing a gene derived from the wax moth Galleria mellonella and expressing the silk protease inhibitor 2 (SPI2). The synthetic SPI2 gene was fused with mGFP-5 to facilitate its detection in plants. Cultivar Velox, which exhibits medium resistance to Phytophthora infestans, was used for transgenosis. The polyclonal antibody confirmed the presence of a protein of expected size in the extracts of transgenic plants. The presence of the fusion protein in resistant plants was confirmed with additional analytical methods. Indirect ELISA showed up to 3 fold increase of protease inhibition in transformed plants compared to the control background level. The tested resistance to late blight showed heterogeneity among transgenic lines, i.e. some lines were more resistant and others were more susceptible than the non-transgenic plants.

Název v anglickém jazyce

Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack

Popis výsledku anglicky

Extracellular serine proteases are often used as a factor of pathogenicity. To improve potato resistance to the pathogens, we prepared plants containing a gene derived from the wax moth Galleria mellonella and expressing the silk protease inhibitor 2 (SPI2). The synthetic SPI2 gene was fused with mGFP-5 to facilitate its detection in plants. Cultivar Velox, which exhibits medium resistance to Phytophthora infestans, was used for transgenosis. The polyclonal antibody confirmed the presence of a protein of expected size in the extracts of transgenic plants. The presence of the fusion protein in resistant plants was confirmed with additional analytical methods. Indirect ELISA showed up to 3 fold increase of protease inhibition in transformed plants compared to the control background level. The tested resistance to late blight showed heterogeneity among transgenic lines, i.e. some lines were more resistant and others were more susceptible than the non-transgenic plants.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

IOBC-Wprs Bulletin

ISSN

1027-3115

e-ISSN

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Svazek periodika

73

Číslo periodika v rámci svazku

Jan 2012

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

7

Strana od-do

61-67

Kód UT WoS článku

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EID výsledku v databázi Scopus

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