Sex chromosomes and associated rDNA form a heterochromatic network in the polytene nuclei of Bactrocera oleae (Diptera: Tephritidae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00380704" target="_blank" >RIV/60077344:_____/12:00380704 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/12:43883622 RIV/00027162:_____/12:#0000925

Výsledek na webu

<a href="http://link.springer.com/article/10.1007/s10709-012-9668-3" target="_blank" >http://link.springer.com/article/10.1007/s10709-012-9668-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10709-012-9668-3" target="_blank" >10.1007/s10709-012-9668-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sex chromosomes and associated rDNA form a heterochromatic network in the polytene nuclei of Bactrocera oleae (Diptera: Tephritidae)

Popis výsledku v původním jazyce

The olive fruit fly, Bactrocera oleae, has a diploid set of 2n = 12 chromosomes including a pair of sex chromosomes, XX in females and XY in males, but polytene nuclei show only five polytene chromosomes, formed by five autosome pairs. We examined the fate of the sex chromosomes in the polytene complements of this species using FISH with the X and Y chromosome-derived probes, prepared by laser microdissection of the respective chromosomes from mitotic metaphases. In polytene nuclei, both probes hybridized strongly to a granular heterochromatic network. The X chromosome probe highlighted most of the granular mass, whereas the Y chromosome probe identified a small compact body of this network. We also examined distribution of rDNA using FISH with an 18SrDNA probe that hybridized exclusively to the sex chromosomes. Our study clearly shows that the granular heterochromatic network of polytene nuclei in B. oleae is formed by the underreplicated sex chromosomes and associated rDNA.

Název v anglickém jazyce

Sex chromosomes and associated rDNA form a heterochromatic network in the polytene nuclei of Bactrocera oleae (Diptera: Tephritidae)

Popis výsledku anglicky

The olive fruit fly, Bactrocera oleae, has a diploid set of 2n = 12 chromosomes including a pair of sex chromosomes, XX in females and XY in males, but polytene nuclei show only five polytene chromosomes, formed by five autosome pairs. We examined the fate of the sex chromosomes in the polytene complements of this species using FISH with the X and Y chromosome-derived probes, prepared by laser microdissection of the respective chromosomes from mitotic metaphases. In polytene nuclei, both probes hybridized strongly to a granular heterochromatic network. The X chromosome probe highlighted most of the granular mass, whereas the Y chromosome probe identified a small compact body of this network. We also examined distribution of rDNA using FISH with an 18SrDNA probe that hybridized exclusively to the sex chromosomes. Our study clearly shows that the granular heterochromatic network of polytene nuclei in B. oleae is formed by the underreplicated sex chromosomes and associated rDNA.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA600960925" target="_blank" >IAA600960925: Evoluční historie pohlavních chromosomů motýlů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genetica

ISSN

0016-6707

e-ISSN

—

Svazek periodika

140

Číslo periodika v rámci svazku

4-6

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

169-180

Kód UT WoS článku

000308968300007

EID výsledku v databázi Scopus

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