First Report of Hop stunt viroid Infecting Hop in Slovenia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00381533" target="_blank" >RIV/60077344:_____/12:00381533 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

First Report of Hop stunt viroid Infecting Hop in Slovenia

Popis výsledku v původním jazyce

Since 2007, symptoms similar to Hop stunt viroid (HSVd) infection have been observed in several hop gardens in Slovenia. Symptoms include stunting, leaf curl, small cones, and dry root rot. In the first year, the incidence varied from 1 to 30% and increased rapidly (by as much as 10%) each subsequent year, mostly along plant rows. For molecular identification of the pathogen, RNA was extracted from leaves and cones of symptomatic and asymptomatic plants from two different hop gardens. Reverse transcription-PCR was carried out using two pairs of specific HSVd primers. Both primer pairs gave a specific PCR product from symptomatic plant tissue, but not from asymptomatic samples. PCR products were subjected to sequencing. Five sequences obtained had 96-99% sequence identity with various HSVd variants from GenBank. To our knowledge, this is the first report of HSVd on hop in Europe. Strict phytosanitary measures have been taken to prevent further spread and to eradicate HSVd infections.

Název v anglickém jazyce

First Report of Hop stunt viroid Infecting Hop in Slovenia

Popis výsledku anglicky

Since 2007, symptoms similar to Hop stunt viroid (HSVd) infection have been observed in several hop gardens in Slovenia. Symptoms include stunting, leaf curl, small cones, and dry root rot. In the first year, the incidence varied from 1 to 30% and increased rapidly (by as much as 10%) each subsequent year, mostly along plant rows. For molecular identification of the pathogen, RNA was extracted from leaves and cones of symptomatic and asymptomatic plants from two different hop gardens. Reverse transcription-PCR was carried out using two pairs of specific HSVd primers. Both primer pairs gave a specific PCR product from symptomatic plant tissue, but not from asymptomatic samples. PCR products were subjected to sequencing. Five sequences obtained had 96-99% sequence identity with various HSVd variants from GenBank. To our knowledge, this is the first report of HSVd on hop in Europe. Strict phytosanitary measures have been taken to prevent further spread and to eradicate HSVd infections.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GCP501%2F10%2FJ018" target="_blank" >GCP501/10/J018: Pospiviroidní patogeneze jako regulační porucha zprostředkovaná malými viroid-specifickými RNA.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů