Organ growth without cell division: somatic polyploidy in a moth, Ephestia kuehniella

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F12%3A00384781" target="_blank" >RIV/60077344:_____/12:00384781 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1139/g2012-060" target="_blank" >http://dx.doi.org/10.1139/g2012-060</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1139/g2012-060" target="_blank" >10.1139/g2012-060</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Organ growth without cell division: somatic polyploidy in a moth, Ephestia kuehniella

Popis výsledku v původním jazyce

Organ growth depends on cell division and cell growth. Here, we present a study on two organs whose growth depends entirely on cell growth, Malpighian tubules and silk glands of Ephestia kuehniella. We determined the number of endocyles required to reachthese stages by Feulgen cytometry. Cells of Malpighian tubules were in the 2C stage in first instar larvae and reached 1024C after 9 endocycles in last instar larvae (1C = 0.45 pg DNA). Silk gland cells already reached a DNA content of 8C-16C in first instar larvae and attained up to 8192C in last instar larvae after a total of 12 endocycles. The nuclei were small and spherical in first instar larvae, but they were huge, flat, and bizarrely branched in last instar larvae. We consider branching as a compensatory adaptation to improve molecular traffic between nucleus and cytoplasm in highly polyploid cells (i) by reducing the mean distance between nucleus and cytoplasm and (ii) by enlarging the surface-to-volume ratio of these nuclei.

Název v anglickém jazyce

Organ growth without cell division: somatic polyploidy in a moth, Ephestia kuehniella

Popis výsledku anglicky

Organ growth depends on cell division and cell growth. Here, we present a study on two organs whose growth depends entirely on cell growth, Malpighian tubules and silk glands of Ephestia kuehniella. We determined the number of endocyles required to reachthese stages by Feulgen cytometry. Cells of Malpighian tubules were in the 2C stage in first instar larvae and reached 1024C after 9 endocycles in last instar larvae (1C = 0.45 pg DNA). Silk gland cells already reached a DNA content of 8C-16C in first instar larvae and attained up to 8192C in last instar larvae after a total of 12 endocycles. The nuclei were small and spherical in first instar larvae, but they were huge, flat, and bizarrely branched in last instar larvae. We consider branching as a compensatory adaptation to improve molecular traffic between nucleus and cytoplasm in highly polyploid cells (i) by reducing the mean distance between nucleus and cytoplasm and (ii) by enlarging the surface-to-volume ratio of these nuclei.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA600960925" target="_blank" >IAA600960925: Evoluční historie pohlavních chromosomů motýlů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Genome

ISSN

0831-2796

e-ISSN

—

Svazek periodika

55

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CA - Kanada

Počet stran výsledku

9

Strana od-do

755-763

Kód UT WoS článku

000311897800002

EID výsledku v databázi Scopus

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