Imbalance in expression of hop (Humulus lupulus) chalcone synthase H1 and its regulators during hop stunt viroid pathogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00393225" target="_blank" >RIV/60077344:_____/13:00393225 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/13:43885633 RIV/14864347:_____/13:#0000494

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jplph.2012.12.006" target="_blank" >http://dx.doi.org/10.1016/j.jplph.2012.12.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jplph.2012.12.006" target="_blank" >10.1016/j.jplph.2012.12.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Imbalance in expression of hop (Humulus lupulus) chalcone synthase H1 and its regulators during hop stunt viroid pathogenesis

Popis výsledku v původním jazyce

Shifts in phenylpropanoid metabolites and changes in petiole coloration were observed during hop stunt viroid (HSVd) pathogenesis of the hop cultivar ?Admiral. Using quantitative RT-PCR, we examined hop lupulin gland-specific genes that have been shown to be involved in phenylpropanoid metabolism, for altered expression in response to infection with two HSVd isolates, HSVd-g and CPFVd. The expression of hop chalcone synthase chs H1 decreased up to 40-fold in infected samples. In addition, a marked changes in the expression of some regulatory factors were also observed. Targeting of the chs_H1 transcript by vd-sRNAs may also contribute to these expression changes. Our data show that HSVd infection causes a significant imbalance in the expression of phenylpropanoid metabolite-affecting genes via a complex mechanism, possibly involving regulatory disorders and direct targeting by vd-sRNA.

Název v anglickém jazyce

Imbalance in expression of hop (Humulus lupulus) chalcone synthase H1 and its regulators during hop stunt viroid pathogenesis

Popis výsledku anglicky

Shifts in phenylpropanoid metabolites and changes in petiole coloration were observed during hop stunt viroid (HSVd) pathogenesis of the hop cultivar ?Admiral. Using quantitative RT-PCR, we examined hop lupulin gland-specific genes that have been shown to be involved in phenylpropanoid metabolism, for altered expression in response to infection with two HSVd isolates, HSVd-g and CPFVd. The expression of hop chalcone synthase chs H1 decreased up to 40-fold in infected samples. In addition, a marked changes in the expression of some regulatory factors were also observed. Targeting of the chs_H1 transcript by vd-sRNAs may also contribute to these expression changes. Our data show that HSVd infection causes a significant imbalance in the expression of phenylpropanoid metabolite-affecting genes via a complex mechanism, possibly involving regulatory disorders and direct targeting by vd-sRNA.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Plant Physiology

ISSN

0176-1617

e-ISSN

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Svazek periodika

170

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

PT - Portugalská republika

Počet stran výsledku

8

Strana od-do

688-695

Kód UT WoS článku

000318755800008

EID výsledku v databázi Scopus

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