Selective elimination/RNAi silencing of FMRF-related peptides and their receptors decreases the locomotor activity in Drosophila melanogaster

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F13%3A00394182" target="_blank" >RIV/60077344:_____/13:00394182 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0016648013002621" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0016648013002621</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ygcen.2013.05.023" target="_blank" >10.1016/j.ygcen.2013.05.023</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Selective elimination/RNAi silencing of FMRF-related peptides and their receptors decreases the locomotor activity in Drosophila melanogaster

Popis výsledku v původním jazyce

Drosophila FMRF-related neuropeptides are functionally unrelated peptides targeting different organs, with different effective thresholds, time-course and expression patterns. They were all suggested to have an effect on the locomotor activity of Drosophila adults. We prepared RNAi knockdowns of the peptides and their specific receptor genes as well as ablated FMRF-positive neurons by inducing apoptosis using the reaper (rpr) gene expression. We show that RNAi silencing of the FaRP and FaRP-receptor genes effectively decreased the startle-induced locomotor activity of flies. Consistantly, the ablation of FMRF-positive neurons by the RS8-Gal4 and/or RS17-Gal4 drivers also significantly decreased fly locomotion.

Název v anglickém jazyce

Selective elimination/RNAi silencing of FMRF-related peptides and their receptors decreases the locomotor activity in Drosophila melanogaster

Popis výsledku anglicky

Drosophila FMRF-related neuropeptides are functionally unrelated peptides targeting different organs, with different effective thresholds, time-course and expression patterns. They were all suggested to have an effect on the locomotor activity of Drosophila adults. We prepared RNAi knockdowns of the peptides and their specific receptor genes as well as ablated FMRF-positive neurons by inducing apoptosis using the reaper (rpr) gene expression. We show that RNAi silencing of the FaRP and FaRP-receptor genes effectively decreased the startle-induced locomotor activity of flies. Consistantly, the ablation of FMRF-positive neurons by the RS8-Gal4 and/or RS17-Gal4 drivers also significantly decreased fly locomotion.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

General and Comparative Endocrinology

ISSN

0016-6480

e-ISSN

—

Svazek periodika

191

Číslo periodika v rámci svazku

SEP 15

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

137-145

Kód UT WoS článku

000323870400015

EID výsledku v databázi Scopus

—